山銀花Lonicerae Flos作為一種常用的藥食兩用中藥,具有清熱解毒、疏散風(fēng)熱的功效,廣泛應(yīng)用于中成藥、食品和保健品。近年來,市場需求的增加使山銀花的成分、藥效和質(zhì)量成為研究焦點(diǎn)。山銀花的質(zhì)量受到基原、產(chǎn)地、批次和加工方式等多種因素的影響,其化學(xué)成分復(fù)雜、不穩(wěn)定,給質(zhì)量控制帶來嚴(yán)峻挑戰(zhàn)。通過系統(tǒng)梳理中國知網(wǎng)(China national knowledge infrastructure,CNKI)與Web of Science(WOS)中山銀花的基原、成分、生物效應(yīng)質(zhì)量評控相關(guān)文獻(xiàn),明確山銀花的變異成分、活性成分,并通過計算變異系數(shù)(coefficient of variation,CV)預(yù)測山銀花可能的I類質(zhì)控指標(biāo)。通過網(wǎng)絡(luò)藥理學(xué)和分子對接技術(shù),將預(yù)測的I類質(zhì)控指標(biāo)與清熱解毒功效相關(guān)聯(lián)。以綠原酸為例,分析了I類質(zhì)控指標(biāo)在山銀花的“基原鑒別-生產(chǎn)過程監(jiān)控-制劑藥效保障”全產(chǎn)業(yè)鏈的參與方式,為后續(xù)山銀花在生產(chǎn)加工以及制劑過程中關(guān)鍵監(jiān)控成分提供參考,也為其質(zhì)量標(biāo)準(zhǔn)優(yōu)化提供理論依據(jù)。

Shanyinhua (Lonicerae Flos), a commonly used medicinal and edible herb, is renowned for its heat-clearing, detoxifying, and wind-dispersing properties, leading to its extensive application in traditional Chinese patent medicines, foods, and health products. In recent years, increasing market demand has made the components, efficacy, and quality of Lonicerae Flos a research focus. However, its quality is influenced by various factors such as botanical origin, geographical location, batch variations, and processing methods, resulting in complex and unstable chemical compositions that pose significant challenges for quality control. By systematically reviewing literature from the China National Knowledge Infrastructure (CNKI) and Web of Science (WOS) concerning the origin, chemical constituents, and bio-effect quality assessment and control of Lonicerae Flos, this study aims to identify its variable components and active constituents. The coefficient of variation (CV) was calculated to predict potential class I marker components for quality control. Network pharmacology and molecular docking techniques were employed to associate these predicted type I markers with the herb’s clearing heat and detoxifying efficacy. Finally, using chlorogenic acid as an example, the study illustrates how class I marker components can be integrated throughout the entire industrial chain of Lonicerae Flos from botanical identification and production process monitoring to efficacy assurance in formulated products. This provides a reference for identifying key components to monitor during production, processing, and formulation, and offers a theoretical basis for optimizing its quality standards.

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國家自然科學(xué)基金項(xiàng)目（81874344）；湖南省重點(diǎn)領(lǐng)域研發(fā)計劃（2023SK2046）；湖南省衛(wèi)生健康高層次人才重大科研專項(xiàng)（R2023139）；湖南省自然科學(xué)基金項(xiàng)目（2023JJ60474）；長沙市自然科學(xué)基金項(xiàng)目（kq2208148，kq2208191）；湖南創(chuàng)新型省份建設(shè)專項(xiàng)（2024RC8110）