目的 研究不同產地藤茶藥材(顯齒蛇葡萄Ampelopsis grossedentata莖葉)貯藏期間有效成分的含量變化,為藤茶藥材的貯藏條件和有效期的制定提供依據(jù)。方法 采用加速試驗和長期試驗法,以藤茶藥材的性狀、水分、二氫楊梅素含量和HPLC-PDA指紋圖譜為考察指標,采用主成分分析法和相關性分析法考察成分變化之間的相關性,并采用紫外法、HPLC-MS質譜及圓二色譜法對未知成分及轉化機制進行解析。結果 在加速試驗9個月過程中,藤茶中二氫楊梅素的含量基本穩(wěn)定,水分有所增加,但仍符合標準要求;楊梅素含量呈升高趨勢,未知峰呈下降趨勢,二者變化具有顯著的相關性,未知峰推測為二氫楊梅素的異構體。在36個月的長期試驗過程中,藤茶水分、二氫楊梅素含量及各特征峰的單位質量峰面積均趨于穩(wěn)定。結論 藤茶常溫條件下,貯藏3年內質量穩(wěn)定,其貯藏過程應避免高溫高濕的環(huán)境。

Objective To study the changes in the content of active ingredients in vine tea (Ampelopsis grossedentata, using stems and leaves as medicinal parts) medicinal materials during storage from different origins, providing a basis for determining the storage conditions and shelf life of vine tea medicinal materials. Methods Accelerated testing and long-term testing methods were utilized, with the traits, moisture content, dihydromyricetin content, and HPLC-PDA fingerprints of vine tea, as well as the content of dihydromyricetin and HPLC-PDA fingerprint patterns as indicators. Principal component analysis and correlation analysis were employed to investigate the correlation between changes in components, and UV spectroscopy, HPLC-MS, and circular dichroism spectroscopy were used to analyze unknown components and conversion mechanisms. Results During the nine-month accelerated testing process, the content of dihydromyricetin in vine tea remained relatively stable, with a slight increase in moisture content, which still met standard requirements. The the area of unknown peaks showed an upward trend, there was a significant correlation between the two changes, and the unknown peaks were speculated to be isomers of dihydromyricetin. During the 36-month long-term testing process, moisture content, dihydromyricetin content, and the peak area per unit mass of each characteristic peak of vine tea all tended to stabilize. Conclusion Under normal temperature conditions, vine tea medicinal materials can maintain stable quality for up to three years. During the storage process, it is important to avoid environments with high temperature and humidity.

R286.2

湖北省技術創(chuàng)新計劃重點研發(fā)專項（2023BCB063）；湖北省技術創(chuàng)新計劃重點研發(fā)專項（2023BCB130）；湖北省技術創(chuàng)新計劃重點研發(fā)專項（2024BCB034）