目的 系統(tǒng)解析七葉樹Aesculus chinensis R2R3-MYB轉(zhuǎn)錄因子家族成員信息,明確其在不同組織器官中的表達(dá)模式,為該類基因在七葉樹中生物學(xué)功能的研究提供參考。方法 利用生物信息學(xué)方法對七葉樹的R2R3-MYB轉(zhuǎn)錄因子家族成員進(jìn)行鑒定,并對其進(jìn)化關(guān)系、基因結(jié)構(gòu)、理化性質(zhì)、染色體定位和基因表達(dá)模式進(jìn)行分析。結(jié)果 在七葉樹全基因組中共鑒定出129條R2R3-MYB基因,分布在20條染色體上,進(jìn)化分析結(jié)果顯示R2R3-MYB家族基因被聚類到34個亞組中,其中S15亞組分布的七葉樹R2R3-MYB成員最多為9個;共線性基因?qū)υ谶M(jìn)化樹的分支距離更近,且遺傳距離較近的基因具有類似的內(nèi)含子-外顯子結(jié)構(gòu);在七葉樹R2R3-MYB基因家族成員中共鑒定出53種不同類型的順式作用元件;對七葉樹不同組織部位的轉(zhuǎn)錄組數(shù)據(jù)分析,結(jié)合系統(tǒng)進(jìn)化分析及七葉皂苷合成關(guān)鍵酶轉(zhuǎn)錄因子結(jié)合位點預(yù)測,推測AcMYB043、AcMYB059、AcMYB022、AcMYB012、AcMYB075、AcMYB111和AcMYB125基因可能參與七葉皂苷生物合成的調(diào)控。結(jié)論 通過對七葉樹全基因組129個七葉樹R2R3-MYB基因進(jìn)行生物信息學(xué)分析,篩選出7個可能參與七葉皂苷生物合成調(diào)控的候選基因,為后續(xù)解析七葉皂苷轉(zhuǎn)錄調(diào)控機(jī)制研究奠定分子基礎(chǔ),對七葉皂苷的合成生物學(xué)研究和高產(chǎn)七葉皂苷七葉樹的新品種培育具有重要指導(dǎo)意義。

Objective To systematically analyze the members of the R2R3-MYB transcription factor family in Qiyeshu (Aesculus chinensis) and their expression patterns in different tissues,, Which provides a reference for the study of the biological function of R2R3-MYB transcription factors in A. chinensis. Methods In this study, bioinformatics methods were used to identify the R2R3-MYB transcription factor family members of A. chinensis, and their evolutionary relationships, gene structure, physicochemical properties, chromosomal localization and gene expression patterns were analyzed. Results The results showed that a total of 129 R2R3-MYB genes were identified in the whole genome of A. chinensis, distributed on 20 chromosomes. The results of evolutionary analysis showed that the R2R3-MYB family genes of A. chinensis were clustered into 34 subgroups, and the maximum number of R2R3-MYB in the S15 subgroup was nine. The collinearity gene pairs were closer on the branches in the phylogenetic tree and the genes with closer genetic distance have similar intron-exon structures. The promoter region of each member contains numerous cis-acting elements. A total of 53 different types of cis-acting elements were identified in the promoter regions of these R2R3-MYB family members. Combined with phylogenetic analysis and prediction of the transcription factors binding sites of key enzymes for escin synthesis, it is speculated that AcMYB043, AcMYB059, AcMYB012, AcMYB022, AcMYB075, AcMYB111 and AcMYB125 genes are likely to be involved in the regulation of escin biosynthesis. Conclusion Through bioinformatics analysis of 129 R2R3-MYB genes on the whole genome of horse chestnut, a total of seven candidate genes were screened. This study lays a molecular foundation for subsequent analysis of the transcriptional regulatory mechanism of escin, and is of great significance for the synthetic biology research of A. chinensis and the cultivation of new varieties with high-yield aescin.

R286.12

國家自然科學(xué)基金項目（82304673）；中國中醫(yī)科學(xué)院科技創(chuàng)新工程項目（CI2023E002）；云南特色植物提取實驗室開放研究項目基金資助（YKKF2024020）