目的 基于序貫代謝方法結(jié)合超高效液相色譜-四極桿-軌道阱-線性離子阱質(zhì)譜(UPLC-Q-OT-qIT MS)聯(lián)用技術(shù),系統(tǒng)分析一枝蒿提取物及其腸代謝、肝代謝和綜合代謝產(chǎn)物,全面表征其入血原型成分及體內(nèi)代謝轉(zhuǎn)化規(guī)律。方法 制備一枝蒿提取物,通過腸灌流收集腸壁代謝、腸道菌群代謝、肝代謝的血漿樣本,ig給藥收集綜合代謝血漿樣本,結(jié)合UPLC-Q-OT-qIT MS對各個樣本所含成分進(jìn)行分析與鑒定。結(jié)果 從一枝蒿提取物中共發(fā)現(xiàn)黃酮類、倍半萜類、有機(jī)酸類等53個化學(xué)成分。在腸代謝樣品中發(fā)現(xiàn)43個原型入血成分和20個代謝產(chǎn)物,肝代謝樣品中發(fā)現(xiàn)28個原型入血成分和20個代謝產(chǎn)物,綜合代謝組樣品中發(fā)現(xiàn)18個原型入血成分和17個代謝產(chǎn)物。結(jié)論 基于序貫代謝研究,系統(tǒng)探究一枝蒿于大鼠體內(nèi)的代謝規(guī)律,鑒定其原型入血成分及代謝產(chǎn)物,揭示不同代謝部位的成分差異及生物轉(zhuǎn)化過程,為明確一枝蒿藥效物質(zhì)基礎(chǔ)及其質(zhì)量標(biāo)準(zhǔn)提升提供科學(xué)依據(jù)與數(shù)據(jù)支持。

Objective To systematically analyze the extract of Artemisia rupestris and its intestinal metabolism, liver metabolism and comprehensive metabolic products based on sequential metabolic method combined with ultra-high performance liquid chromatography-quadrupole-Orbitrap-linear ion trap mass spectrometry (UPLC-Q-OT-qIT MS) joint technology, and fully characterize its blood-entering prototype components and the regularity of in vivo metabolic transformation. Methods The extract of A. rupestris was prepared, and after in vitro intestinal perfusion, plasma samples of intestinal wall metabolism, intestinal flora metabolism and liver metabolism were separately collected. After intragastric administration, comprehensive metabolic plasma samples were collected. The components contained in each sample were analyzed and identified by UPLC-Q-OT-qIT MS. Results A total of 53 chemical components including flavonoids, sesquiterpenes and organic acids, were detected in the extract of A. rupestris. A total of 43 prototype blood-entering components and 20 metabolites were detected in the intestinal metabolism samples, 28 prototype blood-entering components and 20 metabolites were detected in the liver metabolism samples, and 18 prototype blood-entering components and 17 metabolites were detected in the comprehensive metabolic group samples. Conclusion Based on the sequential metabolic method, the metabolic patterns of A. rupestris in rats were systematically analyzed, its prototype blood-entering components and metabolites were identified, and the differences in components among different metabolic sites and the biological transformation process were revealed, providing a scientific basis and data support for clarifying the pharmacodynamic material basis of A. rupestris and improving its quality standards.

R285.61

新疆維吾爾自治區(qū)重點研發(fā)計劃項目（2023B03012-2）；新疆維吾爾自治區(qū)公益性科研院所基本科研業(yè)務(wù)經(jīng)費(fèi)資助項目（KY2025088）；新疆維吾爾自治區(qū)自然科學(xué)基金-杰出青年項目（2021D01E25）