目的 研究潿洲島來源的海洋真菌Penicillium sp. MDW-627產(chǎn)生的次級代謝產(chǎn)物及其促血管生成活性。方法 綜合運用紅外光譜(IR)、紫外吸收光譜(UV)、高分辨質(zhì)譜(HR-ESI-MS)及核磁共振(1D/2D NMR)技術(shù),鑒定化合物的結(jié)構(gòu),并通過圓二色譜(ECD)以及文獻比對確定化合物的絕對構(gòu)型。對化合物進行基于斑馬魚模型的促血管生成活性測試。結(jié)果 分離鑒定了1個新嗜氮酮類化合物以及9個已知化合物:5-Cl-peniazaphilone G(1)、sclerotioramine(2)、(＋)-sclerotiorin(3)、11-epi-geumsanol B(4)、isochromophilone VI(5)、peniazaphilone C(6)、彎孢菌霉素(7)、diaporthelactone(8)、8-hydroxy-6-methoxy-3-methylisocoumarin(9)、異麥芽酚(10)。促血管生成活性結(jié)果顯示化合物5在濃度為40 μmol/L時具有促血管生成活性。結(jié)論 化合物1為新的嗜氮酮類化合物,命名為5-Cl-盤尼阿扎菲酮,化合物5具有促血管生成活性。

Objective To investigate the secondary metabolites produced by the marine-derived fungus Penicillium sp. MDW-627 isolated from Weizhou Island and evaluate their pro-angiogenic activity. Methods The structures of the compounds were elucidated using a combination of infrared spectroscopy (IR), ultraviolet-visible spectroscopy (UV), High-Resolution ElectroSpray Ionization Mass Spectrometry (HR-ESI-MS), and multidimensional nuclear magnetic resonance (1D/2D NMR). The absolute configuration of the compounds was determined by electronic circular dichroism (ECD) spectroscopy in combination with literature comparison. The pro-angiogenic activity of the compounds was assessed using a zebrafish model. Results A new azaphilone derivative and nine known compounds were isolated and identified: 5-Cl-peniazaphilone G (1), sclerotioramine (2), (+)-sclerotiorin (3), 11-epi-geumsanol B (4), isochromophilone VI (5), peniazaphilone C (6), curvularin (7), diaporthelactone (8), 8-Hydroxy-6-methoxy-3-methylisocoumarin (9), isomaltol (10). The pro-angiogenic activity assay revealed that compound 5 exhibited significant vascular growth-promoting effects at a concentration of 40 μmol/L. Conclusion Compound 1 was identified as a new azaphilone derivative, designated as 5-Cl-peniazaphilone, and compound 5 demonstrated significant pro-angiogenic activity.

R284.1

國家自然科學(xué)基金青年項目（82204276）；廣西自然科學(xué)基金資助項目（2025GXNSFAA069557，2019GXNSFBA185002）；廣西高等學(xué)校千名中青年骨干教師培育計劃