目的 研究薰魯香Pistacia lentiscus的化學(xué)成分及其體外抗炎活性。方法 綜合運(yùn)用開(kāi)放硅膠、MCI、ODS、Sephadex LH-20凝膠及半制備型高效液相色譜等技術(shù)進(jìn)行分離,依據(jù)HR-ESI-MS、IR、NMR、ECD等譜學(xué)技術(shù)鑒定化合物結(jié)構(gòu)。采用CCK-8法篩選化合物對(duì)小鼠單核巨噬細(xì)胞RAW264.7的細(xì)胞毒性,通過(guò)脂多糖(lipopolysaccharide,LPS)誘導(dǎo)的RAW264.7細(xì)胞炎癥模型對(duì)更低無(wú)毒性劑量化合物進(jìn)行抗炎活性評(píng)價(jià)。結(jié)果 從薰魯香中分離得到7個(gè)化合物,分別鑒定為13β-hydroxy-3-oxo-1,11-dien-28-oic acid(1)、11α,12α-epoxy-3,4-seco-olean-4(23)-en-13β,28-dihydroxy-3-oic acid(2)、22-oxo-20-taraxasten-3β-ol(3)、3,11-dioxoolean-12-en-28-oic acid(4)、krukovines A(5)、erythrodiol(6)、11-oxoerythrodiol(7),其中化合物1和2為新化合物?；衔?b>4(40 μmol/L)對(duì)RAW264.7細(xì)胞顯示出明顯的細(xì)胞毒性,化合物1～3、5、6對(duì)LPS誘導(dǎo)下RAW264.7細(xì)胞釋放NO具有一定的抑制作用,IC₅₀值在(16.89±0.98)～(27.33±1.25)μmol/L,弱于陽(yáng)性對(duì)照地塞米松[(11.27±2.25)μmol/L]。結(jié)論 化合物1、2為新齊墩果烷型三萜,分別命名為薰魯香三萜酸A(1)和開(kāi)環(huán)薰魯香三萜酸B(2);化合物3、6、7為首次從該植物分離得到;化合物1～3、5、6表現(xiàn)出一定的抗炎活性。

Objective To investigate the chemical constituents of Pistacia lentiscus and their anti-inflammatory activities in vitro. Methods The compounds were isolated and purified by using a various silica gel, ODS, Sephadex LH-20, and prep HPLC, and their structures were elucidated by HR-ESI-MS, IR, NMR. Screening of cytotoxicity of all compounds to RAW264.7 cells using the CCK-8 method, and evaluate the anti-inflammatory activity of low toxicity compounds through the RAW264.7 cell inflammatory model induced by lipopolysaccharide (LPS). Results Seven compounds were isolated from mastic and identified as13β-hydroxy-3-oxo-1,11-dien-28-oic acid (1), 11α,12α-epoxy-3,4-seco-olean-4(23)-en-13β,28-dihydroxy-3-oic acid (2), 22-oxo-20-taraxasten-3β-ol (3), 3,11-dioxoolean-12-en-28-oicacid (4), krukovines A (5), erythrodiol (6), 11-oxoerythrodiol (7). Compound 4 (40 μmol/L) showed significant cytotoxicity to RAW264.7 cells, while compounds 1—3, 5 and 6 exhibited a certain inhibitory effect on the release of NO by RAW264.7 cells induced by LPS with IC₅₀ value ranging from (16.89 ± 0.98) to (27.33 ± 1.25) μmol/L, which were weaker than the positive control dexamethasone (11.27 ± 2.25) μmol/L. Conclusion Compounds 1 and 2 are new oleanane-type triterpenes named mastriterpenoid acid A and secomastriterpenoid acid B, and compounds 3, 6 and 7 were first isolated from the plant. Compounds 1—3, 5 and 6 exhibit certain anti-inflammatory activity.

R284.1

國(guó)家自然科學(xué)基金資助項(xiàng)目（82060774）；天然與仿生藥物研究江西省重點(diǎn)實(shí)驗(yàn)室開(kāi)放課題（2024SSY07051）