目的 構(gòu)建白芷Angelicae Dahuricae Radix藥材、飲片、標(biāo)準(zhǔn)湯劑、流浸膏劑及血清樣本的HPLC特征圖譜，并依據(jù)其質(zhì)量傳遞過程，篩選白芷的質(zhì)量標(biāo)志物（quality markers，Q-Marker）。方法 收集10批白芷藥材，并加工成飲片、標(biāo)準(zhǔn)湯劑、流浸膏劑和血清樣本。采用HPLC技術(shù)，構(gòu)建5類樣本的特征圖譜，通過相似度評價和指標(biāo)成分的含量及轉(zhuǎn)移率分析，篩選白芷的Q-Marker。結(jié)果 白芷樣本（藥材、飲片、標(biāo)準(zhǔn)湯劑、流浸膏劑及血清）的特征圖譜指認(rèn)出8個高相似度的特征峰。在藥材至飲片傳遞中，7種關(guān)鍵成分（歐前胡素、佛手柑內(nèi)酯、白當(dāng)歸素、異歐前胡素、蛇床子素、水合氧化前胡素、精氨酸）的平均轉(zhuǎn)移率超61%，傳遞穩(wěn)定；飲片至標(biāo)準(zhǔn)湯劑傳遞中，香豆素類成分水合氧化前胡素、白當(dāng)歸素、佛手柑內(nèi)酯可以穩(wěn)定傳遞（＞60%），但精氨酸轉(zhuǎn)移率異常高（＞700%），而歐前胡素、異歐前胡素的含量低且轉(zhuǎn)移率＜20%，蛇床子素未檢出；標(biāo)準(zhǔn)湯劑至流浸膏劑傳遞中，精氨酸、水合氧化前胡素、白當(dāng)歸素穩(wěn)定傳遞（＞70%）；流浸膏劑至血清樣本傳遞中，僅精氨酸、水合氧化前胡素、白當(dāng)歸素檢出并傳遞，其余成分未檢出。結(jié)論 結(jié)合白芷藥材、飲片、標(biāo)準(zhǔn)湯劑、流浸膏劑和血清樣本的特征圖譜、含量及轉(zhuǎn)移率變化，篩選出水合氧化前胡素和白當(dāng)歸素可作為白芷的Q-Marker。

Objective To establish the HPLC fingerprint profiles of Baizhi (Angelicae Dahuricae Radix, ADR) medicinal materials, decoction pieces, standard decoctions, fluid extracts, and serum samples, and screen quality markers (Q-Marker) of ADR based on its quality transfer process. Methods A total of ten batches of ADR medicinal materials were collected and processed into decoction pieces, standard decoctions, fluid extracts, and serum samples. The fingerprint profiles of the five types of samples were established by using HPLC technology. The Q-Marker of ADR were screened through similarity evaluation and analysis of the content and transfer rates of indicator components. Results A total of eight high-similarity characteristic peaks were identified in the fingerprint profiles of ADR samples (medicinal materials, decoction pieces, standard decoctions, fluid extracts, and serum). During the transfer from medicinal materials to decoction pieces, the average transfer rates of seven key components (imperatorin, bergapten, byakangelicin, isoimperatorin, osthole, oxypeucedanin hydrate, and arginine) exceeded 61%, indicating stable transfer. In the transfer from decoction pieces to standard decoctions, the coumarin components oxypeucedanin hydrate, byakangelicin, and bergapten were stably transferred (> 60%). However, the transfer rate of arginine was abnormally high (> 700%), while the content of imperatorin and isoimperatorin was low with transfer rates < 20%, and osthole was not detected. During the transfer from standard decoctions to fluid extracts, arginine, oxypeucedanin hydrate, and byakangelicin were stably transferred (> 70%). In the transfer from fluid extracts to serum samples, only arginine, oxypeucedanin hydrate, and byakangelicin were detected and transferred, while the other components were not detected. Conclusion Based on the fingerprint profiles, content, and transfer rate changes of ADR medicinal materials, decoction pieces, standard decoctions, fluid extracts, and serum samples, oxypeucedanin hydrate and byakangelicin were screened as Q-Marker of ADR.

R283.6

國家自然科學(xué)基金資助項目（31860102）；甘肅省委組織部隴原青年創(chuàng)新創(chuàng)業(yè)人才（個人）項目（GSRC-2023-1-4）；甘肅省高校教師創(chuàng)新基金項目（2023A-53）