目的 研究酸模葉蓼Polygonum lapathifolium全草的化學(xué)成分。方法 利用硅膠、Sephadex LH-20和半制備高效液相色譜等分離技術(shù)進(jìn)行分離純化，通過核磁共振波譜、質(zhì)譜、紅外光譜等方法結(jié)合文獻(xiàn)對分離得到的化合物進(jìn)行結(jié)構(gòu)鑒定。采用DPPH法、ABTS法以及羥基自由基清除方法，評價(jià)化合物的抗氧化活性。結(jié)果 從酸模葉蓼醋酸乙酯部位分離得到20個(gè)化合物，分別鑒定為2-甲基-5-n-十一烷基間苯二酚（1）、5-n-dodecylresorcinol（2）、(3S,5R)-dihydroxy-6R,7-megstigmadien-9-one（3）、植物醇（4）、山柰酚（5）、槲皮素（6）、2',4'-二羥基-6'-甲氧基查耳酮（7）、2',6'-二羥基-4'-甲氧基查耳酮（8）、花旗松素（9）、5,7,3',4'-四羥基-3-甲氧基黃酮（10）、β-谷甾醇（11）、豆甾-4-烯-6β-羥基-3-酮（12）、鞣花酸-3,3'-二甲醚（13）、沒食子酸乙酯（14）、methyl 2,4,6-trihydroxybenzoate（15）、棕櫚酸乙酯（16）、9(Z),12(Z)-十八烷二烯酸（17）、硬脂酸甲酯（18）、棕櫚酸（19）和油酸（20）?；衔?b>1～6、9～14、18對2,2-聯(lián)苯基-1-苦基肼基（2,2-diphenyl-1-picrylhydrazyl，DPPH）和2,2-聯(lián)氮二(3-乙基-苯并噻唑-6-磺酸)二銨鹽 [2,2'-azinobis-(3-ethylbenzthia- zoline-6-sulphonate)，ABTS] 自由基或羥基自由基具有較強(qiáng)的清除活性，化合物1清除DPPH自由基的IC₅₀值為0.023 mg/mL。結(jié)論 化合物1為新的間苯二酚類化合物，命名為酸模葉蓼酚A?；衔?b>1～3、13、15為首次從蓼屬植物中分離得到?；衔?b>1～6、9～14、18具有良好的抗氧化活性。

Objective To study the chemical constituents of Polygonum lapathifolium. Methods The compounds were separated and purified by silica gel, Sephadex LH-20 column chromatography and semi-preparative HPLC. The structures were identified by NMR, MS, IR data analyses and literature data comparison. Their antioxidant activity was evaluated by using the DPPH method, the ABTS method and the hydroxyl radical scavenging method. Results A total of 20 compounds were isolated from the ethyl acetate of P. lapathifolium and elucidated as 2-methyl-5-n-undecylresorcinol (1), 5-n-dodecylresorcinol (2), (3S,5R)-dihydroxy-6R,7-megstigmadien-9-one (3), trans-phytol (4), kaempferol (5), quercetin (6), 2',4'-dihydroxy-6'-methoxychalcone (7), 2',6'-dihydroxy-4'-methoxychalcone (8), dihydroquercetin (9), 5,7,3',4'-tetrahydroxy-3-methoxyflavone (10), β-sitosterol (11), stigmaster-4-en-6β-hydroxy-3-one (12), ellagic acid-3,3'-dimethyl ether (13), ethyl gallate (14), methyl 2,4,6-trihydroxybenzoate (15), ethyl palmitate (16), 9(Z),12(Z)-octadecanedioenoic acid (17), methyl stearate (18), palmitic acid (19), oleic acid (20). Compounds 1―6, 9―14, 18 had the stronger DPPH, ABTS or OH radicals scavenging abilities and compound 1 showed significant abilities with IC₅₀value of 0.023 mg/mL. Conclusion Compound 1 is a new resorcinol derivative, and named polygonumol A. Compounds 1―3, 13 and 15 are isolated from this genus for the first time. Compounds 1―6, 9―14, 18 exhibit significant antioxidant capacity.

R284.1

固原市原州區(qū)科技研發(fā)計(jì)劃項(xiàng)目（固原科發(fā)[2024]33號）；固原市“撥改投”科技研發(fā)項(xiàng)目（2024BGTYF01-44）；寧夏自然科學(xué)基金項(xiàng)目（2022AAC02049，2022AAC03297，2023AAC03355）；寧夏重點(diǎn)研發(fā)計(jì)劃項(xiàng)目（2023BEG03061）