目的 研究紅景天Rhodiola rosea內(nèi)生真菌 Penicillium thomii C18的次生代謝產(chǎn)物。方法 采用硅膠和Sephadex LH-20 柱色譜及制備型高效液相色譜（HPLC）進(jìn)行分離純化，利用核磁共振、質(zhì)譜、旋光等譜學(xué)技術(shù)對(duì)化合物進(jìn)行結(jié)構(gòu)鑒定。結(jié)果 從Penicillium thomii C18培養(yǎng)物的醋酸乙酯萃取物中共分離得到9個(gè)聚酮類化合物，包括1個(gè)新的化合物與8個(gè)已知化合物，分別鑒定為 (2'S)-5,7-二羥基-2-(2'-羥基-4'-氧代戊基])-色原酮 [(2'S)-5,7-dihydroxy-2-(2'-hydroxy-4'-oxopentyl)-chromone，1]、spirocitromycetin（2）、異決明酮（isotorachrysone，3）、1,6-二羥基-3-甲基-8-甲酯基(口山)酮[1,6-dihydroxy-3-methyl-8-carbomethoxyxanthone，4]、1,5-二羥基-3-甲氧基-7-甲基-蒽-9,10-二酮 (1,5-dihydroxy-3-methoxy-7-methyl-anthracene-9,10-dione，5）、9-O-methylneuchromenin（6）、neuchromenin（7）、星形酸（asterric acid，8）、星形酸甲酯（methyl asterrate，9）。結(jié)論 化合物1為新的聚酮類化合物，命名為拖姆酮A，化合物3和5為首次從青霉屬中分離得到。

Objective To study the secondary metabolites of the endophyties Penicillium thomii C18 derived from Rhodiola rosea. Methods The secondary metabolites of P. thomii C18 were separated and purified by silica gel, Sephadex LH-20 column chromatography and preparative HPLC. The structures of the isolated compounds were determined by NMR, HR-ESI-MS, polarimetry and etc. Results A total of nine compounds were isolated from the endophytic fungus P. thomii C18. There was one new compound (2'S)-5,7-dihydroxy-2-(2'-hydroxy-4'-oxopentyl)-chromone (1), and eight known compounds spirocitromycetin (2), isotorachrysone (3), 1,6-dihydroxy-3-methyl-8- carbomethoxyxanthone (4), 1,5-dihydroxy-3-methoxy-7-methyl-anthracene-9,10-dione (5), 9-O-methylneuchromenin (6), neuchromenin (7), asterric acid (8), and methyl asterrate (9). Conclusion Compound 1 is a new polyketide named thomiione A. Compounds 3 and 5 are isolated from Penicillium genus for the first time.