目的 研究寧夏枸杞Lycium barbarum 葉的化學(xué)成分及其體外降糖活性。方法 運(yùn)用硅膠、MCI、ODS、和Sephadex LH-20等柱色譜以及反相制備型HPLC等各種現(xiàn)代色譜分離技術(shù)進(jìn)行系統(tǒng)的分離純化。利用波譜學(xué)方法確定化合物結(jié)構(gòu)，并采用胰島素抵抗人肝癌HepG2細(xì)胞模型進(jìn)行體外降糖活性評(píng)價(jià)。結(jié)果 從寧夏枸杞葉95%乙醇提取物的二氯甲烷和醋酸乙酯部位中分離得到17個(gè)降倍半萜類化合物，分別鑒定為黑麥草內(nèi)酯（1）、(3R,6S)-3-hydroxy-2,2,6-trimeth-7-oxabicyclo[4.3.0]non-9-en-8-one（2）、黑麥草素（3）、去氫吐葉醇（4）、3-hydroxy-5,6-epoxy-7-megastigmen-9-one（5）、4,5-dihydroblumenol（6）、蚱蜢酮（7）、3S,5R-dihydroxy-6S,7-megastig-madien-9-one（8）、3,4-dihydroxy-5,7-megastigmadien-9-one（9）、(−)-(3R,4R)-eucomegastigmane B（10）、布盧姆醇B（11）、(6R,9R)-3-酮-α-紫羅蘭醇-9-O-β-D-吡喃葡萄糖苷（12）、byzantionoside B（13）、(6S,9S)-roseoside（14）、(6S,9R)-roseoside（15）、staphylionoside D（16）、柑橘苷A（17）。結(jié)論 化合物2、3和10為首次從枸杞屬中分離得到，化合物2～17為首次從寧夏枸杞葉中分離得到。化合物1～17在10μmol/L濃度時(shí)，可以增加胰島素誘導(dǎo)抵抗HepG2細(xì)胞的葡萄糖消耗量。

Objective To investigate the chemical constituents of the leaves of Lycium barbarum and their hypoglycemic activity in vitro. Methods The compounds were isolated and purified by silica gel, MCI, ODS, Sephadex LH-20 column chromatography and reversed phase preparation HPLC. The structures of the compounds were identified by spectroscopic method, and the hypoglycemic activity in vitro was evaluated by insulin resistance HepG2 cell model. Results A total of 17 norsesquiterpenoids were isolated from the dichloromethane and ethyl acetate part of the 95% ethanol extract of the leaves of L. barbarum, and their structures were determined as loliolide (1), (3R,6S)-3-hydroxy-2,2,6-trimeth-7-oxabicyclo[4.3.0]non-9-en-8-one (2), epiloliolide (3), dehydrovomifoliol (4), 3-hydroxy-5,6-epoxy-7-megastigmen-9-one (5), 4,5-dihydroblumenol (6), grasshopper ketone (7), 3S,5R-dihydroxy-6S,7-megastig-madien-9-one (8), 3,4-dihydroxy-5,7-megastigmadien-9-one (9), (−)-(3R,4R)-eucomegastigmane B (10), blumenol B (11), (6R,9R)-3-oxo-α-ionol-9-O-β-D-glucopyranoside (12), byzantionoside B (13), (6S,9S)-roseoside (14), (6S,9R)-roseoside (15), staphylionoside D (16), citroside A (17). Conclusion Compounds 2, 3, and 10 were isolated from Lycium genus for the first time, while compounds 2―17 were isolated from the leaves of L. barbarum for the first time. Meanwhile, compounds 1―17 could increased the glucose consumption in insulin-resistant HepG2 cells at a concentration of 10 μmol/L.

R284.1

國(guó)家自然科學(xué)基金資助項(xiàng)目（22177027）;河南省高校科技創(chuàng)新團(tuán)隊(duì)支持計(jì)劃（24IRTSTHN039）;河南省科技研發(fā)計(jì)劃聯(lián)合基金（232301420067）