目的 識別酒萸肉特征性成分并檢測其含量。方法 應(yīng)用UPLC-Q-TOF/MS檢測酒萸肉及其生品化學(xué)成分譜，并基于化學(xué)計(jì)量學(xué)方法識別酒萸肉的特征成分。建立基于超高效液相色譜-光電二極管矩陣檢測器（UPLC-PDA）的酒萸肉特征成分含量測定方法，對12批次酒萸肉及其生品的特征成分含量進(jìn)行檢測。結(jié)果 酒萸肉及其生品的化學(xué)成分譜不盡相同，共獲得10個(gè)可表征酒萸肉的特征成分，包括5-羥甲基糠醛、沒食子酸、原兒茶酸、莫諾苷、馬錢苷酸、獐芽菜苷、山茱萸苷、二氫槲皮素、馬錢苷、山茱萸新苷。建立了基于UPLC-PDA的多成分含量測定方法，方法學(xué)考察結(jié)果顯示10個(gè)特征成分線性關(guān)系良好（r≥0.999 7）。與同批次生品比較，酒萸肉中的5-羥甲基糠醛、二氫槲皮素、沒食子酸、馬錢苷、莫諾苷、山茱萸苷和獐芽菜苷7個(gè)成分含量顯著升高，原兒茶酸、馬錢苷酸和山茱萸新苷3個(gè)成分含量顯著降低。結(jié)論 基于化學(xué)計(jì)量學(xué)篩選獲得的特征成分可以區(qū)分酒萸肉及其生品，建立的酒萸肉多成分含量測定方法，線性關(guān)系良好、快速簡便、穩(wěn)定可靠，可為酒萸肉質(zhì)量控制提供參考。

Objective To screen the differential ingredients between crude and wine-processed Corni Fructus and determin their content. Methods An integrated strategy using ultra performance liquid chromatography coupled with tandem quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) and the chemometric approach was applied to compare the global chemical profile of crude and wine-processed Corni Fructus. Then, the main differential ingredients were quantified by UPLC-PDA. Results The chemical profiling of wine-processed Corni Fructus was significantly different. Ten compounds could be considered as characteristic chemical markers for distinguishing crude and wine-processed Corni Fructus, including 5-hydroxymethyl furfuraldehyde (5-HMF), gallic acid, protocatechuic acid, morroniside, loganic acid, sweroside, cornin, dihydroquercetin, loganin and cornoside. A new UPLC-PDA quantitative method for analyzing simultaneously the above ten compounds in wine-processed Corni Fructus was established. The results of methodology investigation showed that the ten components were well linear within the investigation range (r ≥ 0.999 7). Compared with the crude Corni Fructus, the content of seven components were increased, including gallic acid, 5-HMF, loganin, morroniside, cornin, sweroside and dihydroquercetin, and the other three components in wine-processed Corni Fructus were decreased. Conclusion The differential ingredients obtained by chemometric-based approach can be used to distinguish crude and wine-processed Corni Fructus. The determination method of wine-processed Corni Fructus established is accurate and reliable, which can be used for the quality control of Corni Fructus.

R286.2

國家中藥標(biāo)準(zhǔn)化項(xiàng)目（ZYBZH-C-JL-24）