目的 對(duì)不同基原淫羊藿藥材不同部位所含的黃酮類(lèi)成分進(jìn)行比較，為淫羊藿質(zhì)量評(píng)價(jià)及藥用部位的合理選擇提供依據(jù)。方法 采集13批淫羊藿植株地上部分，分為葉片、葉柄、莖3個(gè)部位，對(duì)其HPLC指紋圖譜和朝藿定A、B、C及淫羊藿苷、寶藿苷I 5種黃酮類(lèi)成分進(jìn)行分析，進(jìn)而采用方差分析和中藥色譜指紋圖譜相似度評(píng)價(jià)軟件并結(jié)合聚類(lèi)分析（HCA）對(duì)淫羊藿葉片、葉柄和莖的含量差異進(jìn)行綜合評(píng)價(jià)。結(jié)果 指紋圖譜結(jié)果表明淫羊藿葉片中化學(xué)成分較莖及葉柄中豐富，葉柄與莖化學(xué)成分基本一致；含量測(cè)定結(jié)果表明淫羊藿葉片中5種成分含量均顯著高于葉柄及莖，甚至達(dá)到數(shù)10倍。聚類(lèi)分析結(jié)果也表明葉片與葉柄及莖可明顯區(qū)分開(kāi)來(lái)。結(jié)論 通過(guò)分析淫羊藿不同部位化學(xué)成分差異，明確了淫羊藿葉片、葉柄及莖的質(zhì)量差異，為淫羊藿質(zhì)量控制及藥用部位的確定提供了實(shí)驗(yàn)依據(jù)。

Objective To compare the flavonoids contained in different parts of different botanical origins of Epimedii Folium, and provide a basis for the quality evaluation of Epimedii Folium and the reasonable selection of medicinal parts. Methods The aerial parts of 13 batches of Epimedii Folium were collected and divided into three parts:leaf, petiole and stem. The HPLC fingerprint and content of five flavonoids, including epimedin A, epimedin B, epimedin C, icariin and baohuoside I, were analyzed. Then the analysis of variance and the similarity evaluation software of traditional Chinese medicine chromatographic fingerprint were used. Combined with cluster analysis (HCA), the content differences of flavonoids in leaf, petiole and stem of Epimedii Folium were evaluated. Results The fingerprints showed that the chemical constituents in Epimedii Folium leaves were richer than stems and petioles, and the chemical constituents in petioles and stems were basically the same. The content of five components in leaves was significantly higher than that of petiole and stem, even up to 10 times. Cluster analysis also showed that the leaves were obviously distinct from the petiole and stem. Conclusion The quality differences of Epimedii Folium leaves, petioles and stems were clarified, and this study can provide the scientific evidence for the selection of medicinal parts and quality control of Epimedii Folium.

國(guó)家重大新藥創(chuàng)制項(xiàng)目（2018ZX09301030-008）；江蘇省科教強(qiáng)衛(wèi)醫(yī)學(xué)重點(diǎn)人才項(xiàng)目（ZDRCA2016036）