目的 探討辛芷鼻敏膠囊對變應(yīng)性鼻炎大鼠的血清中白細胞介素-5（IL-5）、白細胞介素-13（IL-13）和鼻黏膜組織中的eotaxin、CCR3、信號轉(zhuǎn)導和轉(zhuǎn)錄激活因子-6（STAT6）基因表達的影響，探討其可能的作用機制。方法 采用卵白蛋白制備變應(yīng)性鼻炎大鼠模型。造模后大鼠隨機分為模型組、辛芷鼻敏膠囊（2.3 g/kg）組、鼻炎康（1.8 g/kg）陽性對照組，每天給藥1次，連續(xù)給藥10 d，另設(shè)對照組。ELISA法檢測各組大鼠血清IL-5、IL-13水平；用原位雜交（ISH）法半定量檢測鼻黏膜中eotaxin、CCR3、STAT6基因的表達。結(jié)果 辛芷鼻敏膠囊組大鼠血清IL-5、IL-13水平和鼻黏膜中STAT6基因表達明顯低于模型組及鼻炎康組（P＜0.05）。結(jié)論 辛芷鼻敏膠囊可能通過下調(diào)STAT6基因表達，抑制大鼠變應(yīng)性鼻炎發(fā)病過程中IL-5、IL-13的水平，達到減少嗜酸性粒細胞浸潤進而改善變應(yīng)性鼻炎癥狀的目的。

Objective To explore the effects of Xinzhi Bimin Capsule (XBC) on the contents of interleukin (IL)-5 and IL-13 in serum and the expression of eotaxin, CCR3, and signal transducers and activators of transcription-6 (STAT6) in nasal mucosa of rat models with allergic rhinitis, and to identify the mechanism of pharmacodynamic action. Methods The allergic rhinitis rat model was established using ovalbumin, and forty rats were randomly divided into four groups, model, normal control, XBC (1.8 g/kg), and Biyankang Tablet (1.8 g/kg, positive control) groups. The rats were administered once daily for 10 d. The contents of IL-5 and IL-6 were detected by ELISA, and the expression of positive cell of eotaxin, CCR3, and STAT6 in nasal mucosa was detected in semi quantity by in situ hybridization (ISH) method. Results The expression of STAT6 in nasal mucosa and the levels of IL-5 and IL-13 in blood serum were markedly lower in XBC group than those in the model and positive control groups (P < 0.05). Conclusion XBC could reduce the expression of eotaxin, CCR3, and STAT6 in nasal mucosa of rats and down-regulate the contents of IL-5 and IL-13 in serum, so as to decrease the eosinophil infiltration and to treat allergic rhinitis in rats.

國家自然科學基金資助項目（30560144）；新疆維吾爾自治區(qū)高校科研計劃重點項目（XJEDU2006I041）