目的 從血清藥理學(xué)的角度，結(jié)合體外溶出度實(shí)驗(yàn)，研究冬蟲夏草用于抗肝纖維化最佳的粉碎度。方法 冬蟲夏草粉碎后分別過(guò)100、150、200、300目篩，制成不同粉碎度的冬蟲夏草粉樣品，結(jié)合腺苷的體外溶出度實(shí)驗(yàn)，采用體外肝纖維化細(xì)胞模型，不同粉碎度的冬蟲夏草粉大鼠ig給藥后，以大鼠含藥血清對(duì)HSC-T6細(xì)胞增殖的影響為指標(biāo)，采用效應(yīng)動(dòng)力學(xué)方法研究不同粉碎度的冬蟲夏草粉對(duì)肝纖維化細(xì)胞的抑制作用。結(jié)果 粉碎度為200～300目冬蟲夏草粉腺苷累積溶出度較好，冬蟲夏草粉含藥血清具有較好的抑制HSC-T6細(xì)胞增殖的作用，200～300目冬蟲夏草粉含藥血清對(duì)HSC-T6抑制的效應(yīng)動(dòng)力學(xué)曲線下面積較大。結(jié)論 基于體外溶出度與效應(yīng)動(dòng)力學(xué)的方法可用于冬蟲夏草治療肝纖維化的粉碎度研究，粉碎度以200～300目最優(yōu)。

Objective To study the optimum particle size of Cordyceps sinensis for liver fibrosis in combination with in vitro dissolution experiment from serum pharmacology. Methods To prepare the powder samples with different grinding degrees, Cordyceps sinensis was crushed through 100-, 150-, 200-, and 300-mesh sieves. The in vitro dissolution of adenosine was measured at different time points. Meanwhile, the powder sample was ig administered to rats, and pharmacodynamic approach was adopted to study the inhibition of medicated serum on HSC-T6 proliferation. Results The accumulative in vitro dissolution of C. sinensis by 200－300 meshes was higher than that of other meshes. Medicated serum could significantly inhibit HSC-T6 cell proliferation. The AUC of HSC-T6 inhibition kinetics of medicated serum crushed to 200－300 meshes was significantly higher than that in other groups. Conclusion The in vitro dissolution and pharmacodynamic method could be used for the study on different particle sizes of C. sinensis for anti-hepatic fibrosis, and 200－300 meshes are the optimal particle size.

國(guó)家新藥創(chuàng)制重大專項(xiàng)（2011ZX09201-201-14，2011ZX09201-201-35）；國(guó)家自然科學(xué)基金資助項(xiàng)目（81073069）