[關(guān)鍵詞]
[摘要]
目的 基于組分結(jié)構(gòu)理論對(duì)不同廠家生產(chǎn)的銀杏葉提取物中黃酮類活性成分進(jìn)行分析比較。方法 采用HPLC法同時(shí)測(cè)定銀杏葉提取物中槲皮素、山柰酚、異鼠李素3種黃酮類成分；利用F檢驗(yàn)考察7個(gè)廠家銀杏葉提取物黃酮類成分相互間的顯著性差異程度。結(jié)果 7個(gè)廠家（A～G）生產(chǎn)的銀杏葉提取物中槲皮素、山柰酚、異鼠李素的質(zhì)量分?jǐn)?shù)差異較大，其中E廠家樣品中槲皮素質(zhì)量分?jǐn)?shù)最高，平均為6.933 1%；D和G兩廠家的樣品中槲皮素質(zhì)量分?jǐn)?shù)分別只為E廠家的56.52%、54.88%；A、B、C、F 4個(gè)廠家樣品中槲皮素質(zhì)量分?jǐn)?shù)分別是E廠家的71.50%、69.99%、67.87%、63.34%。C廠家樣品中山柰酚質(zhì)量分?jǐn)?shù)最高，平均為5.367 0%；而D、G兩廠家樣品中山柰酚質(zhì)量分?jǐn)?shù)分別只有C廠家的66.51%、66.82%；A、B、E、F 4個(gè)廠家樣品中山柰酚質(zhì)量分?jǐn)?shù)分別是C廠家的90.90%、87.03%、67.14%、77.05%。D廠家樣品中異鼠李素質(zhì)量分?jǐn)?shù)最高，平均為1.526 9%，而C、E兩廠家樣品中異鼠李素只有D廠家的40.88%、51.04%；A、B、F、G 4個(gè)廠家的異鼠李素質(zhì)量分?jǐn)?shù)分別是D廠家的70.69%、92.49%、82.49%、80.82%。結(jié)論 市場(chǎng)上銀杏葉制劑療效參差不齊，通過試驗(yàn)比較研究銀杏葉提取物黃酮組分中有效成分的質(zhì)量分?jǐn)?shù)及其配比，發(fā)現(xiàn)存在顯著性差異。
[Key word]
[Abstract]
Abstract: Objective To compare the contents of the active flavonoids in Ginkgo biloba extract (GBE) from different manufactories based on “component structure” theory. Methods The HPLC method was used to determine the contents of quercetin, kaempferol, and isorhamnetin in GBE. The significant differences of flavonoids in GBE from seven manufactories (A－G) were investigated by using F test investigation ingredients. Results The significant differences were found in the contents of quercitin, kaempferol, and isorhamnetin among these GBEs from the seven manufactories. The content of quercitin from the manufactory E was the highest, being 6.93%, whereas the contents of quercitin from the manufactories D and G were the lowest, being 56.52% and 54.88% of that in the manufactory E. In addition, the contents of quercitin from A－C, and F were 71.50%, 69.99%, 67.87%, and 63.34% of that in the manufactory E. The content of kaempferol from the manufactory C was the highest, being 5.367 0%. The contents of kaempferol from the manufactories D and G were 66.51% and 66.82% of that in the manufactory C. In addition, the contents of kaempferol from manufactories A, B, E, and F were 90.90%, 87.03%, 67.14%, and 77.05% of that in the manufactory C, respectively. The content of isorhamnetin from manufactory D was the highest, being 1.526 9%. The contents of isorhamnetin from manufactories C and E were 40.88% and 51.04%. The contents of isorhamnetin from manufactories A, B, F, and G were 70.69%, 92.49%, 82.49%, and 80.82% of that in the manufactory D, respectively. Conclusion The contents of flavonoids in GBEs from the different manufactories have the significant differences. The contents of market effective flavonoid component and content ratios of GBE exist the significant differences, causing that the curative effect of ginkgo preparations are different.
[中圖分類號(hào)]
[基金項(xiàng)目]
江蘇省中醫(yī)藥科技項(xiàng)目（LZ09061）；中藥制藥過程新技術(shù)國家重點(diǎn)實(shí)驗(yàn)室開放基金（SKL2010Z0303）
