目的 觀察水杉總黃酮 (TFM)對(duì)容量超負(fù)荷大鼠心肌肥厚的作用及其對(duì)心室 c- Fos蛋白表達(dá)的影響。方法 采用腹腔動(dòng) -靜脈造瘺建立大鼠容量超負(fù)荷模型,ig給予 TFM,免疫組化測(cè)定心室 c- Fos蛋白的相對(duì)含量。結(jié)果 TFM劑量依賴性地減輕大鼠心臟重量,抑制心室 RNA和蛋白質(zhì)合成,并抑制心室 c- Fos蛋白表達(dá)。結(jié)論 TFM可預(yù)防容量超負(fù)荷大鼠心肌肥厚的形成,抑制心室 c- Fos蛋白表達(dá)可能是其分子機(jī)制之一。

Object To observe the effects of total flavones of Metasequoia glyptostroboides Hu et Cheng (TFM) on volume-overload cardiac hypertrophy and the expression of c-Fos protein in rat. Methods Volume-overload cardiac hypertrophy of rat was induced by aortocaval shunts. The rats were given ig TFM (400, 40 and 4 mg/kg/d). c-Fos protein in the ventricles were measured by immunocytochemical study. Results TFM at the above dosage decreased heart weight and contents of RNA and protein in the myocardium, inhibited the expression of c-Fos protein in the ventricles. Conclusion TFM can prevent volume-overload cardiac hypertrophy in rats. The inhibitory effects on the expression of c-Fos protein may be its mechanism in the molecular level.

國(guó)家自然科學(xué)基金資助課題(NO.39970085)