丹皮酚每天腹腔注射60mg/kg，連續(xù)15d，每天2次，然后結(jié)扎大鼠左降枝冠狀動(dòng)脈45min，解除結(jié)扎恢復(fù)血流灌注60min，復(fù)制大鼠心肌缺血再灌注損傷模型。觀察丹皮酚對(duì)心肌細(xì)胞線粒體膜膽固醇/磷酯的比值、膜Ca⁺⁺－ATP酶活性、膜脂流動(dòng)性及心肌細(xì)胞游離脂肪酸的影響。結(jié)果丹皮酚能降低線粒體膜膽固醇/磷脂的比值，減少心肌細(xì)胞游離脂肪酸含量，改善Ca⁺⁺－ATP酶活性，改善和調(diào)節(jié)線粒體膜脂的流動(dòng)性。表明丹皮酚能明顯改善缺血再灌注損傷心肌線粒體膜的結(jié)構(gòu)和功能，與保護(hù)心肌細(xì)胞膜脂結(jié)構(gòu)有關(guān)。

60mg /kg·d ip, of paeonol were given to rats for 15 days. The myocardial ischemia reperfusion injury model was produced by occluding the left coronary artery and releasing the occlusion in rats. This significantly decreased myocardial Ca++-ATPase activitics and CH/PL radical, FFA content, improved mitochondrial membrane fluidity and kept them away from oxygen free radical damage. These results indicated that paeonol has membrane protective effect on myocardial ischemia rcperfusion injury probably by inhibiting the oxygen free radicals and subsequent lipid peroxidation