摘 要：目的 獲得白花丹參丙酮酸脫羧酶全長(zhǎng)基因，分析該基因在白花丹參不同組織部位，以及缺氧脅迫處理后的該基因表達(dá)差異。方法 利用cDNA文庫(kù)篩選獲得丙酮酸脫羧酶SmPDC基因全長(zhǎng)，利用半定量RT-PCR，分析SmPDC基因在白花丹參不同部位的表達(dá)情況，及缺氧處理?xiàng)l件下的表達(dá)情況。結(jié)果 獲得的SmPDC基因由2 190個(gè)核苷酸組成，編碼605個(gè)氨基酸，蛋白相對(duì)分子質(zhì)量約6.485×104，等電點(diǎn)pI 5.49；半定量RT-PCR檢測(cè)，該基因在丹參的根中表達(dá)量最高，其次是莖和葉；缺氧脅迫處理會(huì)誘導(dǎo)該基因的表達(dá)，隨脅迫時(shí)間延長(zhǎng)表達(dá)量逐漸增加。結(jié)論 白花丹參SmPDC基因是PDC家族新成員，其功能與植物耐缺氧代謝途徑有關(guān)。

Abstract: Objective To obtain the full-length of Salvia miltiorrhiza pyruvate decarboxylase (SmPDC) gene, to analyze the expression differences in various tissues of S. miltiorrhiza after anaerobic stress treatment. Methods The full-length of SmPDC gene was isolated through sequencing cDNA library, and semi-quantitative RT-PCR was used to detect the gene expression levels. Results The full-length of SmPDC cDNA has an open reading frame of 2 190 bp. The deduced amino acid sequence of SmPDC has 605 amino acid residues which form a 64.85 kDa polypeptide with a calculated pI of 5.49. Semi-quantitative RT-PCR indicated that SmPDC gene was expressed at a high level in root, followed by stem and leaf of S. miltiorrhiza. Anaerobic stress could induce the expression of SmPDC gene and the expression was increased with the stress time elongating. Conclusion SmPDC is a new member of the PDC family and plays an important role in anaerobic respiration pathway.

山東省自然科學(xué)基金項(xiàng)目（ZR2010CM063）