間充質(zhì)干細(xì)胞來源外泌體(MSCs-Exo)近年來成為替代間充質(zhì)干細(xì)胞發(fā)揮治療作用的研究熱點(diǎn),良好的外泌體分離和貯存方法是保障獲得的外泌體具有穩(wěn)定的質(zhì)量和可重復(fù)的關(guān)鍵,也是臨床轉(zhuǎn)化的前提。外泌體具有多種分離方式,包括國際金標(biāo)準(zhǔn)的超速離心法,改良后的蔗糖密度梯度離心法、超濾法以及微流控技術(shù)等。貯存溫度和貯存介質(zhì)是影響外泌體生物學(xué)完整性的主要因素。結(jié)合當(dāng)下MSCs-Exo的研究現(xiàn)狀及其分離和貯存條件的研究進(jìn)展進(jìn)行綜述,以期為臨床轉(zhuǎn)化研究提供參考。

Mesenchymal stem cell derived exosomes (MSCs-Exo) has become a research hotpot in the field of replace Mesenchymal Stem Cell (MSCs) on the therapeutic recently. A good method of exosome separation and storage is the key to ensure the stable quality and repeatability of the obtained exosomes, and is also the premise of clinical transformation. Exosomes have a variety of separation methods, including international gold standard ultrafiltration method, improved sucrose density gradient centrifugation method, ultrafiltration method and microfluidic technology. Storage temperature and storage medium are the main factors affecting the biological integrity of exosomes. This article reviews the current research status of MSCs-Exo and their isolation and storage conditions in order to provide reference for translational research.

R965.3

中國醫(yī)學(xué)科學(xué)院醫(yī)學(xué)與健康科技創(chuàng)新工程項(xiàng)目(2019-I2M-5-020);天津市科技計(jì)劃項(xiàng)目:細(xì)胞產(chǎn)品開發(fā)和臨床應(yīng)用的研究(22ZYJDSY00150);天津市科技計(jì)劃項(xiàng)目:細(xì)胞制品的成藥性及轉(zhuǎn)化研究(23ZGCXQY00050)