目的 以注射用益氣復(fù)脈（凍干）生產(chǎn)過程中棄去的固體廢棄物為例，探討生脈類制劑生產(chǎn)過程中紅參、麥冬、五味子固體廢棄物組合（SMGFW）對小鼠免疫功能的影響。方法 將280只SPF級ICR雄性小鼠隨機分成4組：對照組和SMGFW低、中、高劑量（0.86、1.71、3.43 g·kg^-1）組，每組70只。通過刀豆蛋白A（ConA）誘導(dǎo)的小鼠脾淋巴細胞轉(zhuǎn)化實驗、綿羊紅細胞（SRBC）誘導(dǎo)小鼠足跖增厚實驗、抗體生成細胞檢測實驗、半數(shù)溶血值（HC₅₀）測定實驗、碳廓清實驗、小鼠腹腔巨噬細胞吞噬雞紅細胞實驗（半體內(nèi)法）和NK細胞活性測定實驗（乳酸脫氫酶測定法）評價SMGFW增強小鼠免疫功能的作用。結(jié)果 與對照組比較，SMGFW低劑量組小鼠血清抗體水平顯著增加（P<0.001）；SMGFW中劑量組小鼠脾臟指數(shù)顯著增加（P<0.05），小鼠脾淋巴細胞增殖率顯著增加（P<0.001），小鼠足跖差值顯著增加（P<0.001），小鼠溶血空斑數(shù)顯著增加（P<0.001），小鼠血清抗體水平顯著增加（P<0.001），小鼠碳廓清吞噬指數(shù)顯著增加（P<0.05），小鼠巨噬細胞對雞紅細胞吞噬百分率顯著增加（P<0.001），吞噬指數(shù)顯著增加（P<0.001），小鼠NK細胞活性顯著增加（P<0.001）；SMGFW高劑量組小鼠體質(zhì)量增加值顯著降低（P<0.01），小鼠足跖差值顯著增加（P<0.001），小鼠溶血空斑數(shù)顯著增加（P<0.001），小鼠血清抗體水平顯著增加（P<0.001），小鼠巨噬細胞對雞紅細胞吞噬百分率顯著增加（P<0.05），吞噬指數(shù)顯著增加（P<0.01），小鼠NK細胞活性顯著增加（P<0.01）。結(jié)論 生脈類制劑生產(chǎn)過程中紅參、麥冬、五味子固體廢棄物組合具有增強小鼠免疫功能的作用。

Objective Carry out research on solid waste combination of Panax ginseng, Ophiopogon japonicus and Schisandra chinensis (SMGFW) in the production process of biogenic preparations, taking the solid waste discarded during the production of Yiqi Fumai Lyophilized Injection product for injection as an example, the effect of different doses of SMGFW on the immune function of mice was discussed. Methods A total of 280 male scrubs with SPF-grade ICR were randomly divided into four groups: control group, low, medium and high doses of SMGFW(0.86, 1.71, 3.43g·kg^-1), 70 in each group. The effect of SMGFW in enhancing the immune function of mice was evaluated by canavalin A (ConA)-induced mouse splenic lymphocyte transformation experiment, sheep red blood cell (SRBC) -induced mouse plantar thickening experiment, antibody-producing cell detection experiment, half-hemolytic value (HC₅₀) determination experiment, carbon clearance experiment, mouse intraperitoneal macrophage phagocytosis chicken erythrocytes experiment (semi-in vivo method) and NK cell activity determination experiment (lactate dehydrogenase assay). Results Compared with the control group, The serum antibody level of mice in the low-dose group of SMGFW increased significantly (P< 0.001), the spleen index of mice in the medium-dose group of SMGFW increased significantly (P< 0.05), the value-added rate of splenic lymphocytes in mice increased significantly (P< 0.001), the plantar difference of mice increased significantly (P< 0.001), the number of hemolytic spots in mice increased significantly (P< 0.001), the serum antibody level of mice increased significantly (P< 0.001), the carbon clearance phagocytosis index of mice increased significantly (P< 0.05), and the percentage of macrophages engulfing chicken erythrocytes by mouse macrophages increased significantly (P< 0.001), the phagocytosis index increased significantly (P< 0.001), the activity of mouse NK cells increased significantly (P< 0.001), the body weight gain value of mice in the high-dose group of SMGFW was significantly decreased (P< 0.01), the plantar difference of mice increased significantly (P< 0.001), the number of hemolytic empty spots in mice was significantly increased (P< 0.001), the serum antibody level of mice was significantly increased (P< 0.001), the percentage of mouse macrophages to engulf chicken red blood cells was significantly increased (P< 0.05), the phagocytic index was significantly increased (P< 0.01), and the activity of mouse NK cells was significantly increased (P< 0.01). Conclusion SMGFW has the effect of enhancing the immune function of mice.