目的 采用超高效液相色譜-四極桿飛行時(shí)間質(zhì)譜(UPLC-Q-TOF-MS)對速效救心丸的化學(xué)成分進(jìn)行研究。方法 采用 Agilent Poroshell 120 EC C₁₈柱(150 mm×3.0 mm,2.7 μm),以0.1%甲酸水-乙腈為流動(dòng)相梯度洗脫,體積流量為0.8 mL·min^-1(分流比 1:2);采用 Dual AJS ESI 源在正、負(fù)離子模式下分別采集數(shù)據(jù)。構(gòu)建化合物庫,根據(jù)化合物精確相對分子質(zhì)量及二級碎片離子信息結(jié)合參考文獻(xiàn)數(shù)據(jù),鑒定速效救心丸的主要化學(xué)成分。結(jié)果 從速效救心丸中分離和鑒定出90 個(gè)化學(xué)成分,其中苯酞類化合物 47 個(gè),有機(jī)酸類化合物 11 個(gè),生物堿類化合物 13 個(gè),萜類化合物 4 個(gè),其他類化合物15 個(gè)。結(jié)論 該方法快速、準(zhǔn)確、便捷,為闡明速效救心丸的藥效物質(zhì)基礎(chǔ)及進(jìn)一步研究其體內(nèi)代謝過程提供了科學(xué)的數(shù)據(jù)支撐和方法參考。

Objective The chemical constituents of Suxiao Jiuxin Pills (SJP) were studied by ultra-high-performance liquid chromatography coupled with quadruple time of flight mass spectrometry (UPLC-Q-TOF-MS). Methods Agilent Poroshell 120 EC C₁₈ column (150 mm × 3.0 mm, 2.7 μm) was used for gradient elution with 0.1% formic acid aqueous solution and acetonitrile as mobile phases. The volume flow rate was 0.8 mL·min^-1 (split ratio 1: 2). Dual AJS ESI source was used to collect data in positive and negative ion modes. A compound library was established. According to the precise relative molecular weight and secondary fragment ion information of compounds, the main chemical constituents of SJP were identified with reference data. Results 90 constituents were identified from SJP, including 47 phthalides, 11 organic acids, 13 alkaloids, four terpenoids and 15 other compounds. Conclusion The method is rapid, accurate and convenient, which provides scientific data support and method reference for elucidating the pharmacodynamic substance basis of SJP and further studying its metabolism process in vivo.

R284.1

北京市自然科學(xué)基金(7214284);國家自然科學(xué)基金(82173957,81903766)