目的 確定并驗(yàn)證以細(xì)胞表面標(biāo)志物CD54作為評(píng)價(jià)指標(biāo)的光致敏體外評(píng)價(jià)方法。方法 將THP-1細(xì)胞與多種光致敏劑、光刺激劑、皮膚致敏劑、皮膚刺激劑和陰性受試物分別孵育，在光照射或避光處理后，用流式細(xì)胞儀測(cè)定細(xì)胞表面標(biāo)志物CD54和CD86的表達(dá)水平并統(tǒng)計(jì)分析，進(jìn)一步確定具體的評(píng)價(jià)指標(biāo)，并對(duì)該方法的準(zhǔn)確性、特異性、靈敏度、重復(fù)性進(jìn)行驗(yàn)證。結(jié)果 19種光致敏劑中有15種引起照射組THP-1細(xì)胞表達(dá)CD54的平均熒光強(qiáng)度（MFI）較非照射組顯著增加（P<0.05、0.01） ，且照射組細(xì)胞表達(dá)CD54的相對(duì)熒光強(qiáng)度（RFI）值均在1.5以上。光刺激劑經(jīng)光照射后也可引起細(xì)胞表達(dá)CD54的MFI顯著增加（P<0.01） ，但經(jīng)過(guò)預(yù)照射處理后，CD54的表達(dá)水平較直接照射組顯著下降（P<0.01）。未經(jīng)光照射條件下，皮膚致敏劑即可引起THP-1細(xì)胞表達(dá)CD54和CD86的MFI比對(duì)照組顯著增加（P<0.01） ，光照后CD54的表達(dá)反而略有下降。在光照和避光條件下，皮膚刺激劑、陰性受試物（乳酸）均未引起細(xì)胞表達(dá)CD54或CD86的顯著性變化。以CD54為評(píng)價(jià)指標(biāo)的THP-1細(xì)胞光致敏評(píng)價(jià)方法檢測(cè)光致敏劑的準(zhǔn)確性、特異性和靈敏度分別是85.2%、100%和78.9%，具有良好的重復(fù)性。結(jié)論 確定了THP-1細(xì)胞光致敏評(píng)價(jià)方法的細(xì)胞表面標(biāo)志物評(píng)價(jià)指標(biāo)為CD54，判定標(biāo)準(zhǔn)為：（1）光照后THP-1細(xì)胞表達(dá)CD54的MFI較照射前顯著性增加；（2）光照后THP-1細(xì)胞表達(dá)CD54的RFI≥1.5；（3）當(dāng)上述條件均滿(mǎn)足時(shí)，對(duì)受試物進(jìn)行預(yù)照射處理，結(jié)果仍然滿(mǎn)足前2條標(biāo)準(zhǔn)。

Objective To identify and validate an in vitro photoallergy evaluation method with cell surface markers as research indicators. Methods THP-1 cells were incubated with a variety of photoallergy agents, photoirritants, skin sensitizers, skin irritant and negative subject respectively. After solar radiation or non-radiation treatment, the expression changes of cell surface markers CD54 and CD86 were detected by flow cytometry and analysed to confirm the specific evaluation indicators and to validate the accuracy, specificity, sensitivity and repeatability of the method. Results Among the 19 photoallergy agents, 15 induced a significant increase in the mean fluorescence intensity (MFI) of CD54 expressed in THP-1 cells in the irradiated groups compared with the nonirradiated groups (P < 0.05 and 0.01), and the relative fluorescence intensity (RFI) of CD54 in THP-1 cells in the irradiated groups were all above 1.5. The photoirritants also induced the significant increase in CD54 MFI of THP-1 cells after solar irradiation (P < 0.01), but the expression level of CD54 was significantly decreased after pre-irradiation treatment compared with direct irradiation group (P < 0.01). Without solar radiation, the expression levels of CD54 and CD86 in THP-1 cells incubated with the two skin sensitizers were significantly increased compared with control group (P < 0.01), while the expression of CD54 was slightly decreased after irradiation. Both skin irritants and negative subject did not cause the significant change in CD54 and CD86 expression under solar irradiation and in the dark conditions. In this study, the accuracy, specificity and sensitivity of the photoallergy evaluation method using THP-1 cells with CD54 as the evaluation index was 85.2%, 100% and 78.9% respectively, and the method had good repeatability. Conclusion The cell surface marker evaluation index of THP-1 cell photoallergy evaluation method was determined as CD54, and the evaluation criteria were as follows:(1) MFI of THP-1 cells expressing CD54 increased significantly after irradiation compared with before irradiation, (2) The RFI of CD54 expression in THP-1 cells after irradiation was ≥ 1.5, (3) When all the above conditions are met, the test materials are pre-irradiated, and the results still meet the first two standards.

R965.1

國(guó)家“重大新藥創(chuàng)制”科技重大專(zhuān)項(xiàng)資助項(xiàng)目“創(chuàng)新藥物非臨床安全性評(píng)價(jià)研究關(guān)鍵技術(shù)”（2018ZX09201017）