目的 從腸道菌群角度探討酸棗仁湯對(duì)慢性睡眠剝奪小鼠肝功能的影響。方法 C57BL/6雄性小鼠隨機(jī)分為對(duì)照組、模型組和酸棗仁湯組。采用強(qiáng)迫運(yùn)動(dòng)睡眠剝奪法建立14 d的慢性睡眠剝奪模型，酸棗仁湯組在睡眠剝奪同時(shí)給予酸棗仁湯，對(duì)照組正常飼養(yǎng)，不作處理。觀察造模前后小鼠體質(zhì)量和攝食量變化；收集糞便樣本，提取糞便基因組DNA，實(shí)時(shí)熒光定量PCR（qRT-PCR）檢測特定腸道菌的差異；同時(shí)收集血清樣本，試劑盒檢測丙氨酸轉(zhuǎn)氨酶（ALT）和總膽紅素（TBIL）的活性，進(jìn)而指示各組肝功能的變化。結(jié)果 與對(duì)照組比較，模型組小鼠的攝食量顯著增加（P<0.05），體質(zhì)量顯著下降（P<0.05），給予酸棗仁湯顯著增加睡眠剝奪小鼠的進(jìn)食量（P<0.05）。與對(duì)照組比較，睡眠剝奪小鼠的TBIL結(jié)果未見明顯差異；而模型組ALT明顯升高（P<0.05），同時(shí)給予酸棗仁湯可逆轉(zhuǎn)ALT的升高（P<0.05）。qRT-PCR結(jié)果顯示，模型組小鼠糞便中大腸桿菌屬和產(chǎn)氣莢膜梭菌與對(duì)照組比較顯著增加（P<0.05），而雙歧桿菌屬、羅氏菌屬、乳酸菌屬與對(duì)照組比較顯著降低（P<0.05）。同時(shí)給藥可以逆轉(zhuǎn)特定腸道菌大腸桿菌屬、產(chǎn)氣莢膜梭菌和乳酸菌屬的變化（P<0.05），對(duì)雙歧桿菌屬、羅氏菌屬?zèng)]有顯著影響。睡眠剝奪小鼠糞便擬桿菌門/厚壁菌門比有下降趨勢，酸棗仁湯組該趨勢升高，沒有顯著性差異。結(jié)論 酸棗仁湯可能通過改善腸道菌群，緩解慢性睡眠剝奪導(dǎo)致的肝損傷。

Objective To investigate the effect of Suan-Zao-Ren Decoction (SZRD) on liver function in mice with chronic sleep deprivation from the perspective of gut microbiota. Methods C57BL/6 mice were randomly divided into three groups:control group, model group, SZRD group. A 14-day chronic sleep deprivation model was established by using the forced exercise sleep deprivation method. SZRD was given once a day during the 14-day sleep deprivation. The changes of body weight and food intake of mice before and after modeling was observed. Fresh fecal samples were collected for each mouse at the end of the experiment. Fecal genomic DNA was extracted to analyze the gut microbiota by real-time quantitative PCR (qRT-PCR). Serum samples were collected, and the activity of ALT and TBIL indicating changes in liver function were detected. Results The average food intake of all sleep-deprived mice was significantly increased (P<0.05), while the weight has no obvious increase (P<0.05) compared to the control mice. SZRD treatment further increased the food intake of sleep-deprived mice (P<0.05). Compared with control group, there was no significant difference in TBIL activity in sleep-deprived mice. ALT activity was significantly increased in model group (P<0.05), and SZRD treatment reversed the increase in ALT activity (P<0.05). qPCR results showed that E. coli and Clostridium perfringens in the feces of model group were significantly increased compared with the control group (P<0.05), while Bifidobacteria, Roseburia and Lactobacillus were significantly decreased (P<0.05). Meanwhile, SZRD treatment reversed the changes of E. coli, Clostridium perfringens and Lactobacillus (P<0.05), but had no effect on the changes of Bifidobacteria and Roseburia. The ratio of Bacteroidetes to Firmicutes in the feces of sleep-deprived mice decreased, SZRD treatment increased it. And the changes had no statistical significance. Conclusion SZRD can alleviate liver injury caused by chronic sleep deprivation by improving gut microbiota.

R285.5;R289

國家自然科學(xué)基金面上項(xiàng)目（81274114）