目的 研究丹蒽醌對乳腺癌細胞MCF-7增殖及侵襲轉移的抑制作用，并探討其作用機制。方法 CCK-8法檢測不同濃度（10、20、40、60、80、100 μmol/L）的丹蒽醌作用24、48 h對乳腺癌細胞MCF-7增殖能力的影響；Transwell實驗考察丹蒽醌對乳腺癌細胞MCF-7侵襲轉移能力的影響。實時熒光當量PCR（RT-qPCR）法檢測c-Myc、Cyclin D1 mRNA的表達水平；Western blotting法檢測p-AMPKα、ACC、c-Myc、Cyclin D1蛋白的表達水平。結果 不同濃度的丹蒽醌作用不同時間對乳腺癌細胞MCF-7增殖均具有抑制作用；20、40 μmol/L的丹蒽醌能夠明顯的抑制乳腺癌細胞MCF-7的侵襲轉移，同時能夠激活p-AMPKα的表達，抑制ACC、c-Myc、Cyclin D1等基因的表達，從而抑制乳腺癌細胞MCF-7增殖。結論 丹蒽醌對乳腺癌細胞MCF-7增殖及侵襲轉移具有抑制作用，其作用機制可能與激活AMPK信號通路抑制c-Myc、Cyclin D1等增殖相關基因表達有關。

Objective To study the inhibitory effect of dantron on MCF-7 proliferation, invasion and metastasis of breast cancer cells, and to explore its mechanism. Methods CCK-8 assay was used to detect the effect of dantron at different concentrations (10, 20, 40, 60, 80, 100 μmol/L) on MCF-7 proliferation of breast cancer cells for 24 and 48 h. Transwell assay investigated the effect of dantron on the invasion and metastasis of MCF-7 in breast cancer cells. Real-time fluorescence equivalent PCR (RT-qPCR) used to detect the expression levels of c-Myc、Cyclin D1 mRNA, and Western blotting were used to detect the protein expression levels of p-AMPKα, ACC, c-Myc and Cyclin D1. Results Different concentrations of dantron inhibited the proliferation of MCF-7 in breast cancer cells at different time, and 20, 40 μmol/L dantron can significantly inhibited the invasion and metastasis of MCF-7 in breast cancer cells, activate the expression of p-AMPKα, inhibited the expression of ACC, c-Myc, Cyclin D1 genes, and thereby inhibit the proliferation of MCF-7 in breast cancer cells. Conclusion Dantron can inhibit the proliferation, invasion and metastasis of MCF-7 in breast cancer cells, and its mechanism may be related to the activation of AMPK signaling pathway to inhibit the expression of cMyc, Cyclin D1 proliferation-related genes.

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