目的 比較3種不同粒徑注射用姜黃素脂質(zhì)體（CLI）引起的過(guò)敏反應(yīng)，并判斷可能的過(guò)敏反應(yīng)類型。方法 將平均粒徑約為70、100、130 nm的CLI （劑量為20 mg/kg，以姜黃素計(jì)）分別尾iv給予大鼠，分別于注射前，注射后10 min、7 d，眼眶采血，分離血清，ELISA法檢測(cè)注射前后血清中過(guò)敏相關(guān)因子——補(bǔ)體C3a、C3b和C5a，組胺和抗體IgE、IgG、IgM的含量變化。將平均粒徑約為100 nm的CLI和抗過(guò)敏藥地塞米松（預(yù)處理2 h，2 mg/kg）聯(lián)用給予大鼠，觀察血清中補(bǔ)體C3a、C5a和組胺的含量變化。結(jié)果 給予3種粒徑的CLI，血清中補(bǔ)體C3a、C3b、C5a和組胺含量均明顯升高，與給藥前比較差異具有統(tǒng)計(jì)學(xué)意義（P<0.01）；血清中抗體IgE、IgG、IgM的含量均無(wú)明顯變化。預(yù)先給予地塞米松，在注射CLI前后C3a和C5a的含量無(wú)明顯變化，組胺含量極低。結(jié)論 3種不同粒徑CLI在體內(nèi)引起的過(guò)敏反應(yīng)一致，且可能是補(bǔ)體激活相關(guān)的假性過(guò)敏反應(yīng)。

Objective To compare the allergic reactions caused by curcumin liposmes injection (CLI) with different sizes, and analysis the possible type of the allergic reaction caused by those.Methods Curcumin liposomes injection with an average diameter of about 70, 100, and 130 nm were iv administered to rats in tail, and then the concentration of allergy-related factors including complement C3a, C3b and C5a, histamine and antibody IgE, IgG, IgM in serum originated from orbital blood were detected by ELISA before injection and 10 min and 7 d after the injection. Rats were treated with CLI with an average particle size of 100 nm and an anti-allergy drug dexamethasone (pretreated for 2 h, 2 mg/kg). The contents of complement C3a, C5a and histamine in serum were observed.Results Three sizes of CPI could cause the obvious increase of complement factor activation and histamine release, the difference was statistically significant compared with that before administration (P< 0.01). However, there was no significant change in the contents of IgE, IgG and IgM in serum.. Pretreated with dexamethasone, the content C3a and C5a had no obvious change before and after the injection of CLI, the content of histamine was very low.Conclusion The allergic reactions caused by the three sizes of CPI is consistent with each other, which may be the complement activated related pseudo allergic reactions.

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