目的 考察大劑量ig輔酶Q10對(duì)小鼠中樞神經(jīng)系統(tǒng)的影響，為臨床安全性評(píng)價(jià)提供實(shí)驗(yàn)依據(jù)。方法 將小鼠隨機(jī)分為溶媒對(duì)照組、紫蘇油對(duì)照組、陽(yáng)性對(duì)照組（氯丙嗪或地西泮）和輔酶Q10低、中、高劑量組（1.5、3.0和6.0 g/kg，相當(dāng)于臨床等效劑量的75、150和300倍），每組12只，雌雄各半，40 mL/kg單次ig給藥。直接觀察小鼠一般行為活動(dòng)；轉(zhuǎn)棒法觀察小鼠運(yùn)動(dòng)協(xié)調(diào)能力；Anymaze動(dòng)物行為學(xué)視頻分析系統(tǒng)觀察小鼠自發(fā)活動(dòng)和與閾下劑量戊巴比妥鈉的協(xié)同作用。結(jié)果 與溶媒對(duì)照組、紫蘇油對(duì)照組比較，輔酶Q10 3個(gè)劑量組對(duì)小鼠一般行為活動(dòng)、自發(fā)活動(dòng)、運(yùn)動(dòng)協(xié)調(diào)能力和與閾下劑量戊巴比妥鈉的協(xié)同作用均無(wú)顯著差異。結(jié)論 大劑量ig輔酶Q10對(duì)小鼠中樞神經(jīng)系統(tǒng)未見明顯毒性作用。

Objective To investigate the effect of high-dose coenzyme Q10 on the central nervous system in mice, and to provide experimental basis for clinical safety evaluation.Methods Mice were randomly divided into vehicle control group, perillartine control group, positive control group (chlorpromazine or diazepam) and coenzyme Q10 low, medium and high dose groups (1.5, 3.0 and 6.0 g/kg, equivalent to 75, 150, and 300 times of clinical dosage, respectively). The corresponding drugs were ig given to mice with the volume of 40 mL/kg. The general behavior of mice was observed directly, the motor coordination ability was observed by rotating stick method, and Anymaze animal behavior video analysis system was used to observe the spontaneous activity of mice and synergistic reaction with sub-threshold dose of pentobarbital sodium.Results There were no significant differences in the general behavioral activity, and the number of spontaneous activity times, mean resident time, and ratio of sleeping were found in all coenzyme Q10 groups, compared with the vehicle and perillartine control groups.Conclusion High dose of coenzyme Q10 has no significantly toxic effect on the central nervous system in mice, which could provide a reliable experimental basis for further medication study and clinical application of high-dose coenzyme Q10.