目的 使用電噴霧質(zhì)譜技術(shù)，研究3種氨基酸（D-賴氨酸、L-谷氨酰胺和L-酪氨酸）作為手性選擇探針對(duì)格列美脲和格列美脲異構(gòu)體的識(shí)別效應(yīng)。方法 分別將3種氨基酸與格列美脲及其順式異構(gòu)體溶液混合，用電噴霧/離子阱質(zhì)譜提取非共價(jià)結(jié)合的復(fù)合物，在二級(jí)質(zhì)譜中通過(guò)碰撞誘導(dǎo)解離（CID）方法給予不同的碎裂能量，考察碎片離子的豐度從而進(jìn)行格列美脲及其順式異構(gòu)體的識(shí)別。結(jié)果 L-酪氨酸、D-賴氨酸和L-谷氨酰胺對(duì)格列美脲及其順式異構(gòu)體均有識(shí)別效應(yīng)，識(shí)別率分別為1.61、2.92和2.17。結(jié)論 以3種手性氨基酸作為立體異構(gòu)區(qū)分探針，首次實(shí)現(xiàn)了格列美脲和格列美脲順式異構(gòu)體的質(zhì)譜法快速識(shí)別。

Objective Chiral recognition for the glimepiride and glimepiride-cis-isomer by applying three amino acid (D-Lysine, L-Glutamine and L-Tyrosine) as chiral probes based on electrospray ionization mass spectrometry (ESI-MS) was achieved. Methods glimepiride/glimepiride-cis-isomer solutions were mixed with three amino acid solutions. The complex was extracted by ESI-MS and then the fragmentation abundance was investigated applying collision induced dissociation (CID) by MS/MS, which is the basis of chiral recognition for glimepiride and glimepiride-cis-isomer. Results Chiral recognition effect was achieved with the recognition rate (R) 1.61, 2.92 and 2.17 for D-Lysine, L-Glutamine and L-Tyrosine respectively.Conclusion 3 kinds of chiral amino acids were used as probes to distinguish between stereoisomers, and rapid identification of glimepiride and glimepiride cis isomer by mass spectrometry come true for the first time.

中國(guó)食品藥品檢定研究院青年基金（2014A8）