目的 探討藏紫菀總黃酮對高原缺氧小鼠抗氧化能力及細胞凋亡的影響。方法 采用常壓密閉實驗篩選藏紫菀總黃酮最佳抗缺氧劑量;40只小鼠隨機分為對照組、模型組、乙酰唑胺(200 mg/kg)組和藏紫菀總黃酮(500 mg/kg)組,模擬海拔8000 m高原環(huán)境,減壓缺氧12 h,測定小鼠腦組織中的過氧化氫酶(CAT)、谷胱甘肽過氧化物酶(GSH-Px)、還原型谷胱甘肽(GSH)和超氧化物歧化酶(SOD)活性,Western blotting法檢測Nrf-2、SOD、Bcl-2和Bax蛋白表達。結(jié)果 常壓密閉實驗中,藏紫菀總黃酮500 mg/kg劑量組小鼠的存活時間最長,為最佳給藥劑量;與對照組比較,缺氧后小鼠腦組織中SOD、CAT、GSH和GSH-Px活性均顯著降低,Nrf-2蛋白表達顯著升高,SOD蛋白表達和Bc1-2/Bax比值顯著降低 (P<0.01);經(jīng)藏紫菀總黃酮預(yù)處理后,小鼠腦組織中SOD、CAT、GSH和GSH-Px活性均顯著升高,Nrf-2、SOD蛋白表達和Bcl-2/Bax比值顯著升高(P<0.05、0.01)。結(jié)論 藏紫菀總黃酮提高高原缺氧小鼠腦組織抗氧化能力,降低細胞凋亡。

Objective To study the effects of total flavonoids from Aster souliei (ASF) on the expression of hypoxia-associated and apoptosis-associated proteins in brain of mice under hypobaric hypoxia condition. Methods The best dose of ASF to antihypoxia was determined by closed normobaric hypoxia experiment. Forty BALB/c mice were randomly divided into control group, model group, acetazolamide (200 mg/kg) group, and ASF (500 mg/kg) group. The mice were exposed to a simulated high altitude of 8 000 m for 12 h. After hypoxic exposure, mice were sacrificed and the activities of catalase (CAT), supersxide dismutase (SOD), glutathione (GSH), and reduced glutathione tablets (GSH-Px) in brain were determined. Nrf-2, SOD, Bax, and Bcl-2 were detected by Western blotting. Results Compared with control group, the CAT, SOD, GSH, and GSH-PX activity in model group significantly decreased. In addition, the expression of Nrf-2 increased, SOD and Bc1-2/Bax ratio decreased (P < 0.01). Prior administration of ASF effectively increased CAT, SOD, GSH, and GSH-Px activity as well as the expression of Nrf-2, SOD, and Bc1-2/Bax ratio(P < 0.05, 0.01). Conclusion ASF can improve the ability of anti-oxidant enzymes, alleviate oxidative stress as well as inhibit myocardial apoptosis of mice under hypobaric hypoxia condition.

全軍醫(yī)藥衛(wèi)生科研基金課題(CLZ11JA06);甘肅省自然科學基金(1107RJZA100)