目的 研究蓬子菜活性成分香葉木苷抗急性血瘀作用及其作用機(jī)制。方法 采用sc鹽酸腎上腺素復(fù)合冰水浴制備大鼠急性血瘀模型,ig給予不同劑量香葉木苷(45、90、180 mg/kg),阿司匹林為陽(yáng)性藥,應(yīng)用血液流變測(cè)試儀、全自動(dòng)血沉儀檢測(cè)血液流變學(xué)指標(biāo):全血黏度、血漿黏度、紅細(xì)胞比容、血沉及紅細(xì)胞聚集指數(shù);采用試劑盒法檢測(cè)血漿炎癥因子C反應(yīng)蛋白(CRP)、腫瘤壞死因子-α(TNF-α)、白細(xì)胞介素-1β(IL-1β)水平。結(jié)果 與模型組比較,香葉木苷能不同程度降低全血黏度、血漿黏度、紅細(xì)胞比容、血沉及紅細(xì)胞聚集指數(shù),明顯改善血瘀大鼠血液流變學(xué)指標(biāo);顯著降低炎癥因子CRP、TNF-α和IL-1β水平。結(jié)論 香葉木苷有效改善急性血瘀大鼠血液流變學(xué)異常,減少炎癥因子釋放,從而治療急性血瘀及靜脈炎癥,改善微循環(huán)。

Objective To study the influence of diosmin which is one active component abstracted from Galium verum on blood rheology of rat model and investigate the mechanism. Methods Apply epinephrin sc injection and ice water bath to make rat model of blood stasis syndrome. Rats were administered with diosmin (45, 90, and 180 mg/kg) and aspirin as a positive drug. Then make a study that how diosmin ameliorated the hemorheology by assaying the indexes of blood rheology and tetrachoric including whole blood viscosity, plasma viscosity, hematocrit, erythrocyte sedimentation rate and erythrocyte aggregation index using hemeorheology instrument and automatic ESR analyzer. Meantime, we made an approach to the subject that the influence of diosmin on the relationship between blood stasis and inflammation by assaying CRP, TNF-α, and IL-1β. Results Diosmin can improve the indexes of hemorheology obviously. It also can reduce the content of inflammatory factors. Conclution Diosmin can improve the abnormality of hemorheology, and further, it processes the properties of curing inflammation of vein and improving microcirculation by reducing the discharge of inflammatory factors effectively.

國(guó)家自然科學(xué)基金項(xiàng)目(201281274034);教育部博士點(diǎn)博導(dǎo)類基金項(xiàng)目資助(20112327110006)