目的 研究復(fù)方鴉膽子油脂質(zhì)體凍干粉的性質(zhì)，建立其質(zhì)量評價(jià)方法。方法 考察復(fù)方鴉膽子油脂質(zhì)體的形態(tài)、粒徑分布、Zeta電位，采用TLC法對其定性鑒別，并采用溶劑萃取法測定脂質(zhì)體包封率。同時(shí)，通過UV和GC的方法測定其有效成分的含量。結(jié)果 復(fù)方鴉膽子油脂質(zhì)體凍干粉色澤均勻，質(zhì)地細(xì)膩，形態(tài)飽滿，其平均粒徑為170.3 nm，多分散系數(shù)（PDI）為0.258，Zeta電位為?32.1 mV，帶負(fù)電，脂質(zhì)體pH為5.05。TLC鑒別中檢出蟾酥、鴉膽子油斑點(diǎn)清晰可見。脂質(zhì)體凍干粉的平均包封率為91.90%，吲哚類生物堿的平均質(zhì)量分?jǐn)?shù)為5.19 mg/g，油酸的平均質(zhì)量分?jǐn)?shù)為79.08 mg/g，亞油酸的平均質(zhì)量分?jǐn)?shù)50.41 mg/g。脂質(zhì)體的平均過氧化值為0.030 5。結(jié)論 上述方法適用于復(fù)方鴉膽子油脂質(zhì)體凍干粉的質(zhì)量評價(jià)，可有效反映其各方面的性質(zhì)。

Objective To study the properties of liposomes freeze-powder of compound Brucea Javanica oil (LFP-CBO), and to establish the quality evaluation methods. Methods The form, particles distribution, and Zeta potential of LFP-CBO were investigated, TLC was used to identify the chemical composition, the encapsulation efficiency was determined by solvent extraction method, and the active ingredients was determined by UV and GC. Results The mean particle size of LFP-CBO was 170.3 nm with uniform color, fine texture, and round shape, and the polydisperse index (PDI) was 0.258. The Zeta potential was ?32.1 mV with negative charge, and the pH value was 5.05. The spots of B. Javanica and Veneum Bufonis were clear on TLC. The average encapsulation efficiency of the optimal formulation was 91.9%. The concentration of indole alkaloids, oleic acid, and linoleic acid was 5.19, 79.08, and 50.41 mg/g, and the peroxide value of LFP-CBO was 0.030 5. Conclusion The methods above are suitable for the quality evaluation of LFP-CBO, and could effectively demonstrate its properties.