目的 研究馬尾松花粉多糖PPM60-A及其硫酸酯化物SPPM60-A對(duì)大鼠動(dòng)脈平滑肌細(xì)胞 [Ca²⁺]_i調(diào)控及增殖的影響。方法 常規(guī)水提醇沉法制備馬尾松花粉多糖，Sephacryl S-400HR色譜分離得PPM60-A，氯磺酸-吡啶法得硫酸酯化物SPPM60-A，酯化度為1.28。酶解法分離制備大鼠動(dòng)脈平滑肌細(xì)胞，測(cè)定酯化前后多糖對(duì)其胞內(nèi) [Ca²⁺]_i和細(xì)胞增殖的影響。結(jié)果 PPM60-A和SPPM60-A均可以降低 [Ca²⁺]_i，抑制高K⁺和去甲腎上腺素（NE）誘導(dǎo)的鈣離子升高，降低高K⁺引起的鈣離子水平上升，對(duì)NE誘導(dǎo)的血管主動(dòng)脈平滑肌細(xì)胞增殖具有顯著的抑制作用。PPM60-A作用效果好于SPPM60-A。結(jié)論 PPM60-A及SPPM60-A均能抑制細(xì)胞外Ca²⁺內(nèi)流，抑制血管平滑肌細(xì)胞增殖。

Objective To explore the effects of polysaccharide PPM60-A from masson pine (Pinus massoniana) pollen and its sulfated derivative SPPM60-A on [Ca²⁺]_i regulation and proliferation of arterial smooth muscle cells from rats. Methods Polysaccharide (PPM60-A) was extracted by hot water, classified by 60% ethanol from masson pine pollen and purified by Sephacryl S-400HR. Sulfated polysaccharide (SPPM60-A) was obtained from PPM60-A modified by chlorosulfonic acid-pyridin method and its esterification degree is 1.28. The original generation of vascular smooth muscle cells (VSMCs) was obtained by enzyme digestion with collagenae from artery of rats. The effects of PPM60-A and SPPM-60A on [Ca²⁺]i regulation and cell proliferation were determined. Results Both PPM60-A and SPPM60-A could decrease the [Ca²⁺]_i significantly, inhibit the rising of calcium ion concentration induced by KCl and noradrenalin (NE); reduce the calcium ion level pretreated by KCl; inhibit the VSMCs proliferation induced by NE. Conclusion PPM60-A and SPPM60-A could inhibit the influx of calcium ion and the proliferation of VSMCs.

山東省自然科學(xué)基金資助項(xiàng)目（Y2008D13）