目的 觀察、追蹤毛冬青抑制口腔常見致齲菌（變形鏈球菌和具核梭桿菌）的有效部位和成分。方法 通過系統(tǒng)溶劑萃取、大孔樹脂及硅膠柱色譜分離等方法以及質(zhì)譜和核磁共振等鑒定技術(shù)，結(jié)合液體二倍稀釋法，研究毛冬青不同部位及單體對(duì)變形鏈球菌和具核梭桿菌的最低抑菌濃度（MIC），1%玉潔純作為陽(yáng)性對(duì)照藥。結(jié)果 毛冬青三萜總皂苷和冬青素對(duì)兩種主要口腔致病菌均具有一定的抑制作用，特別對(duì)變形鏈球菌具有顯著地抑制效果。結(jié)論 毛冬青三萜總皂苷和冬青素對(duì)變形鏈球菌具有很強(qiáng)的抑制作用，具有開發(fā)成為抗齲齒的保健牙膏或藥品的潛力。

Objective To study and explore the inhibitory effects of the active fractions and compounds from Ilex pubescens on the growth of two major oral pathogens Fusobacterium nucleatum (F.n.) and Streptococcus mutans (S.m.) in vitro. Methods The I. pubescens extract was prepared by organic solvent method and then was separated by macroporous resin and silica gel column chromatograph methods. Sequently, the compounds were identified by the mass spectromety (MS) and nuclear magnetic resonance (NMR) methods. Finally, F.n. and S.m. were chosen as the experimental bacterial strains. The liquid media two-fold dilution method was used to study the minimum inhibitory concentration (MIC) of active fractions and compounds from I. pubescens with 1% triclosan as the positive control. Results Both the total triterpenoid saponins and ilexgenin A showed the effects on the two oral pathogens for some extent, and especially had significant inhibitory activity against S.m. Conclusion The total triterpenoid saponins and ilexgenin A show the significant inhibitory effect on S.m., and may develop into the toothpastes or drugs with anti-caries function.

江蘇省自然科學(xué)青年基金（BK2012378）；江蘇省普通高校研究生科研創(chuàng)新計(jì)劃項(xiàng)目（CX09B_284Z）