目的 研究蒽貝素對(duì)雄性大鼠血漿性腺激素水平、附睪精子質(zhì)量、睪丸組織結(jié)構(gòu)的影響，探討蒽貝素降低雄性大鼠生育能力的作用機(jī)制。方法 給雄性性成熟大鼠sc蒽貝素銨鹽溶液30 d，末次給藥后2 h，測(cè)定血漿性激素水平；檢查附睪精子質(zhì)量；觀察睪丸組織結(jié)構(gòu)。結(jié)果 與對(duì)照組比較，給藥30 d后，蒽貝素高、中劑量（40、20 mg/kg）組大鼠血漿睪酮、黃體生成素、卵泡刺激素水平、附睪精子活率、精子密度明顯降低，組間差異有統(tǒng)計(jì)學(xué)意義（P＜0.05），附睪精子畸形率和頂體完整率無(wú)明顯變化，顯微鏡下見(jiàn)睪丸組織呈萎縮性病變，曲精細(xì)管扁平、塌陷，管內(nèi)精母細(xì)胞減少，附睪精細(xì)管內(nèi)精子稀少。結(jié)論 蒽貝素抑制丘腦-垂體-性腺軸活動(dòng)，降低雄性大鼠血漿性激素水平，引起睪丸萎縮和附睪精子活率、精子密度降低，使雄性大鼠生育能力下降。

Objective To study the effect of embelin on plasma sex hormone levels, epididymis sperm quality, testicular tissue structure, and its mechanism of reducing fertility action in male rat. Methods Sexually-mature rat received the hypodermic injection of embelin salt of ammonia, once daily, for 30 d. Plasma sex hormone levels were determined, epididymis sperm quality was checked up, and testicular tissue structure was observed 2 h after the last hypodermic injection of embelin. Results After hypodermic injection of embelin for 30 d, rat plasma levels of testosterone, luteinizing hormone, follicle-stimulating hormone, survival sperm rate, and sperm density in epididymis were decreased significantly, compared with control group (P < 0.05), no changes in sperm deformity rate and acrosome intact rate were found in all embelin-treated groups; convoluted seminiferous tubules were flat, collapsed, or atrophic pathological changes, decreased spermatogonium was observed in convoluted seminiferous tubule of testicular tissue, extremely low sperm counts were observed too in seminiferous tubule of epididymis under the microscope in high- (40 mg/kg) and mid-(20 mg/kg) dose embelin-treated groups. Conclusion Embelin inhibits the activity of hypothalamus-pituitary-sex gland axis, decreases plasma sex hormone level, leads to atrophy in testicular tissue, and decreases survival sperm rate and sperm density in epididymis, resulting in decreased fertility in male rats.

廣西自然科學(xué)基金項(xiàng)目（2013GXNSFAA019261）；廣西醫(yī)療衛(wèi)生重點(diǎn)科研課題（重2012057）；廣西中藥質(zhì)量標(biāo)準(zhǔn)研究重點(diǎn)實(shí)驗(yàn)室主任基金（桂中重科201103）