目的 建立可靠穩(wěn)定的視網(wǎng)膜新生血管動(dòng)物模型，為探究視網(wǎng)膜新生血管疾病的發(fā)生機(jī)制和治療方法奠定模型基礎(chǔ)。方法 通過(guò)建立氧誘導(dǎo)視網(wǎng)膜新生血管小鼠模型，采用ADP酶染色和視網(wǎng)膜連續(xù)切片法，定量評(píng)估視網(wǎng)膜新生血管生成情況。結(jié)果 模型組小鼠左眼球ADP酶染色結(jié)果顯示，視乳頭周?chē)梢?jiàn)大片無(wú)灌注區(qū)，并見(jiàn)血管迂曲、擴(kuò)張、變形，視網(wǎng)膜周邊部可見(jiàn)大量新生血管生成；右眼球連續(xù)切片發(fā)現(xiàn)模型組小鼠有許多明顯的血管內(nèi)皮細(xì)胞核突破內(nèi)界膜，并且內(nèi)界膜下的細(xì)胞增殖，排列紊亂，并見(jiàn)視網(wǎng)膜表面或視網(wǎng)膜前有新生血管芽。結(jié)論 成功建立氧誘導(dǎo)視網(wǎng)膜新生血管小鼠模型，并且可作為探究視網(wǎng)膜新生血管疾病發(fā)生機(jī)制和治療方法的可靠動(dòng)物模型。

Objective To establish a stable and reliable animal model of retinal neovascularization and to provide a model foundation for further investigation on its mechanism and treatment. Methods Oxygen-induced retina new vessels mouse model were established, and retinal neovascularization was evaluated by ADPase staining and retina sect serial sections. Results ADPase staining showed a pattern of pathological retinal neovascularization such as non-perfused areas, tortuous, dilated blood vessels and deformation in discus opticus of model mice, and retinal neovascularization in retina perimeter. The serial HE staining sections confirmed many conspicuous vascular endothelial cell breakthrough internal limiting membrane, cell proliferation, rank disorder, new vascular bud in retinal surface. Conclusion The oxygen-induced retinal neovascularization mouse model is successfully set up, which could be a reproducible model for the research of mechanism and treatment of retinal neovascularization.