目的：研究治咳川貝枇杷滴丸對豚鼠的鎮(zhèn)咳、平喘作用。方法：采用豚鼠離體氣管平滑肌收縮實驗和組胺引喘的活體動物實驗對治咳川貝枇杷滴丸的平喘作用進行評價。在離體實驗中測定藥物對乙酰膽堿引起的氣管平滑肌收縮的收縮曲線，計算收縮百分率；在整體動物實驗中測定給藥后豚鼠的延喘時間用來表征藥物平喘作用的強弱。同時采用枸櫞酸誘發(fā)豚鼠咳嗽模型，通過記錄引咳后5 min內的咳嗽次數(shù)，觀察治咳川貝枇杷滴丸的鎮(zhèn)咳作用。結果：治咳川貝枇杷滴丸能有效減少豚鼠的咳嗽次數(shù)，顯著延長咳嗽潛伏期；對豚鼠離體氣管收縮的抑制效果較好；對組胺致喘豚鼠的哮喘潛伏期有明顯的延長作用。結論：治咳川貝枇杷滴丸具有良好的鎮(zhèn)咳、平喘藥理活性，為藥物的臨床應用和進一步開發(fā)提供了依據(jù)。

Objective: To study antitussive and anti-asthmatic effect of Zhike Chuanbei Pipa Diwan (ZCPD) in guinea pigs. Methods: Guinea pig models of isolated trachea in vitro and hitamine-induced asthma in vivo were used to investigate the anti-asthmatic effect of ZCPD. In vitro experiment, concentration-response curves of contractile isolated trachea induced by acetylcholine (Ach) were obtained and the concentration producing 50% of the maximal response was determined. In vivo experiment, the latent period of animals after administration was uesd to demonstrate the anti-asthmatic effect. Meanwhile, the aminal cough model induced by citric acid was developed to evaluate the antitussive effect. Qualified guinea pigs were divided into five groups randomly: high, medium, low concentration ZCPD groups, pentoxyverine positive control group, and control group. The number of cough during three minutes was recorded after the cough was induced. Results: ZCPD decreased the frequency of cough and prolonged the latency of induced cough. Morever, it significantly inhibited Ach-induced trachea contractions and prolonged the latent period of asthma by hitamine in guinea pigs. Conclusion: ZCPD has remarkable antitussive and anti-asthmatic effects, which provides the evidence for clinical application and further development.