目的：比較狹窄冠狀動(dòng)脈導(dǎo)致心肌缺血模型犬在麻醉和清醒狀態(tài)下的差異，及藥物治療的差異。方法：在無(wú)菌條件下給犬心外膜安置心電電極、血壓導(dǎo)管、信號(hào)發(fā)射器并分離冠狀動(dòng)脈前降支，放置動(dòng)脈壓迫環(huán)。手術(shù)恢復(fù)期后，動(dòng)物按正交設(shè)計(jì)給予處置：分別在清醒與麻醉狀態(tài)下狹窄冠狀動(dòng)脈，進(jìn)行模型組和硝酸甘油治療組的觀察。主要監(jiān)測(cè)心外膜心電圖ST段、血壓、心率、體溫。結(jié)果與結(jié)論：麻醉犬心率明顯高于清醒犬，冠脈狹窄后麻醉及清醒犬心電圖ST段均明顯升高；硝酸甘油治療的效果，清醒犬較麻醉犬起效快，維持時(shí)間長(zhǎng)。

Objective: Using model of myocardial ischemia by coronary artery stenosis with artery rings, the difference between anesthetized and conscious dogs, as well as drug treatment, was compared. Methods: Under sterile conditions, electrode of epicardial ECG, catheter of blood pressure, and the signal transmitter were imbedded in Beagle dogs, and the artery rings were placed on anterior descending coronary artery. After surgery, the coronary artery stenosis was made in anesthetized or conscious state according to orthogonal design, the therapeutic effect of nitroglycerin or vehicle was observed. Data of epicardial ECG ST-segment, blood pressure, heart rate, and body temperature were acquired and analyzed. Results and Conclusion: Heart rate was higher in anesthetic dogs than that in conscious dogs. Epicardial ECG ST-segment was increased both in conscious and anesthetic dogs significantly after coronary artery stenosis. Therapeutic effects of nitroglycerin provide faster onset of action and longer time in conscious dogs compared with anesthetic dogs.

國(guó)家科技支撐計(jì)劃項(xiàng)目（NO.2007BAI41B02）、系列化、國(guó)際化的國(guó)家生物醫(yī)藥國(guó)際創(chuàng)新園新藥研發(fā)綜合性大平臺(tái)建設(shè)項(xiàng)目（2009ZX09301-008）