目的 采用多指標(biāo)成分定量聯(lián)合PCA、OPLS-DA和CRITIC-TOPSIS法評(píng)價(jià)臭靈丹草質(zhì)量差異。方法 選取臭靈丹草中金絲桃苷、木犀草素、芹菜素、洋艾素、金腰乙素、3,4-O-二咖啡酰奎寧酸、4,5-O-二咖啡酰奎寧酸、3,5-O-二咖啡酰奎寧酸、臭靈丹二醇、冬青葉豚草酸、臭靈丹酸、水溶性浸出物、總灰分和酸不溶性灰分為質(zhì)量評(píng)價(jià)指標(biāo)，以PCA和OPLS-DA法對(duì)15批臭靈丹草進(jìn)行分組，篩選質(zhì)量差異標(biāo)志物；以CRITIC法計(jì)算各指標(biāo)權(quán)重，采用TOPSIS法構(gòu)建臭靈丹草綜合質(zhì)量評(píng)價(jià)模型。結(jié)果 不同產(chǎn)地臭靈丹草中14個(gè)指標(biāo)含量差異較大；OPLS-DA分析可將15批臭靈丹草按照不同產(chǎn)地分為3類(lèi)，篩選出臭靈丹酸、3,5-O-二咖啡酰奎寧酸、洋艾素、冬青葉豚草酸和金腰乙素為質(zhì)量差異標(biāo)志物；CRITIC-TOPSIS法結(jié)果顯示15批樣品綜合質(zhì)量評(píng)價(jià)指標(biāo)的相對(duì)接近度最大值為0.632 1，最小值為0.392 7；云南產(chǎn)臭靈丹草樣品排序靠前。結(jié)論 PCA、OPLS-DA和CRITIC-TOPSIS法可用于臭靈丹草的質(zhì)量差異評(píng)價(jià)，并且不同產(chǎn)地臭靈丹草藥材質(zhì)量存在差異。

Objective To evaluate the quality difference of Laggerae Herba based on multi-index component quantitation combined with PCA, OPLS-DA, and CRITIC-TOPSIS method. Method The contents of 14 quality evaluation indexes, including hyperoside, luteolin, apigenin, artemitin, chrysosplenetin B, 3,4-O-dicaffeoylquinic acid, 4,5-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid, pterodondiol, ilicic acid, pterodontic acid, water soluble extract, total ash, and acid insoluble ash were selected. PCA and OPLS-DA methods were used to group 15 batches of Laggerae Herba, and the quality difference markers were screened. The CRITIC method was used to calculate the weight of each index, and the TOPSIS method was used to construct the comprehensive quality evaluation model of Laggerae Herba. Results The contents of 14 indexes in Laggerae Herba from different producing areas were quite different. OPLS-DA analysis could divide 15 batches of Laggerae Herba into three categories according to different producing areas. Pterodontic acid, 3,5-O-dicaffeoylquinic acid, artemitin, ilicic acid, and chrysosplenetin B were selected as quality difference markers. The results of CRITIC-TOPSIS method showed that the maximum relative proximity of the comprehensive quality evaluation indexes of 15 batches of samples was 0.632 1, and the minimum value was 0.392 7. The rank of Laggerae Herba samples from genuine producing areas Yunnan was front. Conclusion PCA, OPLS-DA, and CRITIC-TOPSIS can be used to evaluate the quality difference of Laggerae Herba, and there are differences in the quality of Laggerae Herba from different producing areas.

R284

河南省醫(yī)學(xué)科技攻關(guān)計(jì)劃聯(lián)合共建項(xiàng)目（LHGJ20230989）