目的 探究2-O-葡萄糖基白及苷對糖尿病小鼠腎臟的保護作用及其對Toll樣受體4/核轉(zhuǎn)錄因子-κB/NOD樣受體蛋白3（TLR4/NF-κB/NLRP3）通路的影響。方法 將C57BL/6小鼠隨機分為對照組、模型組、厄貝沙坦組、2-O-葡萄糖基白及苷組，每組10只。檢測小鼠體質(zhì)量、腎質(zhì)量、腎指數(shù)；通過HE染色、PAS染色、Masson染色觀察腎臟病理形態(tài)；透射電鏡觀察腎臟超微結(jié)構(gòu)；檢測小鼠血清糖代謝指標(biāo)[胰島素（INS）、空腹血糖、糖化血紅蛋白（GHb）]、腎功能指標(biāo)[血尿素氮（BUN）、血肌酐（SCr）和尿白蛋白（ALB）]、炎性因子［白細胞介素（IL）-1β、IL-6、腫瘤壞死因子-α（TNF-α）］水平以及蛋白免疫印跡法檢測腎組織中TLR4、NF-κB p65、p-NF-κB p65和NLRP3蛋白的表達水平。結(jié)果 2-O-葡萄糖基白及苷干預(yù)后，腎組織病理形態(tài)改善，間質(zhì)纖維化程度減輕，基底膜厚度變薄，空腹血糖降低（P＜0.05）；BUN、SCr、ALB水平降低（P＜0.05）；IL-1β、IL-6、TNF-α水平降低（P＜0.05）；TLR4、NLRP3和p-NF-κB p65/NF-κB p65蛋白表達水平顯著降低（P＜0.05）。結(jié)論 2-O-葡萄糖基白及苷能夠減輕糖尿病小鼠腎臟損傷狀態(tài)，調(diào)節(jié)血糖，改善腎功能，減輕炎癥反應(yīng)，其機制可能與抑制TLR4/NF-κB/NLRP3通路有關(guān)。

Objective To investigate the renal protective effect of 2-O-glucosyl bletilla striata glycoside on diabetic mice, and its effect on TLR4/NF-κB/NLRP3 pathway. Methods C57BL/6 mice were randomly divided into control group, model group, irbesartan group, and 2-O-glucosyl bletilla striata glycoside group, with 10 mice in each group. Body weight, kidney weight, and kidney index were measured sequentially. Renal pathological morphology was assessed using HE staining, PAS staining, and Masson staining, while renal ultrastructure was examined via transmission electron microscopy. Serum glucose metabolism indices (INS, fasting blood glucose, GHb), renal function indices (BUN, SCr, ALB), and levels of inflammatory factors (IL-1β, IL-6, TNF-α) were measured. The expression levels of TLR4, NF-κB p65, p-NF-κB p65, and NLRP3 proteins in renal tissue were assessed using Western blotting. Results After 2-O-glucosyl bletilla striata glycoside intervention, the pathological morphology of renal tissue improved, the degree of interstitial fibrosis was alleviated, and the thickness of basement membrane became thinner, fasting blood glucose decreased (P < 0.05). BUN, SCr, and ALB levels were decreased (P < 0.05), IL-1β, IL-6, and TNF-α levels were decreased (P < 0.05). TLR4, NLRP3 and p-NF-κB p65/NF-κB p65 protein expressions were decreased (P < 0.05). Conclusion 2-O-glucosyl bletilla striata glycoside can alleviate renal damage in diabetic mice, regulate blood sugar, improve renal function, and reduce inflammatory response, and its mechanism may be related to the inhibition of TLR4/NF-κB/NLRP3 pathway.

R285.5;R286.7

云南省科技廳科技計劃項目（202101AZ070001-046）