[關(guān)鍵詞]
[摘要]
目的 基于miR-26a-5p/WNT5A信號通路探討天麻素防治陣發(fā)性房顫大鼠的作用機(jī)制。方法 大鼠隨機(jī)分為對照組、模型組、天麻素(16.665、66.660 mg/kg)組、胺碘酮組、天麻素+inhibitor NC組、天麻素+miR-26a-5p inhibitor組,每組12只。檢測房顫誘發(fā)時(shí)間、房顫持續(xù)時(shí)間、左心室分?jǐn)?shù)縮短(LVFS)、左心室射血分?jǐn)?shù)(LVEF)、左心房直徑、左心房面積的變化;Masson染色檢測心房肌組織纖維化;免疫組化染色檢測心房肌組織α-平滑肌肌動蛋白(α-SMA)、I型膠原(Collagen I)陽性表達(dá)面積;qRT-PCR檢測心房肌組織miR-26a-5p水平;Western blotting檢測心房肌組織WNT5A表達(dá)。結(jié)果 與模型組比較,天麻素組心房肌組織纖維化程度減輕,房顫誘發(fā)時(shí)間延長,房顫持續(xù)時(shí)間縮短,LVFS、LVEF增加,左心房直徑、左心房面積減少,心房肌組織中miR-26a-5p水平升高,α-SMA、Collagen I陽性表達(dá)面積及WNT5A表達(dá)降低(P<0.05)。miR-26a-5p inhibitor逆轉(zhuǎn)了天麻素對陣發(fā)性房顫大鼠心房結(jié)構(gòu)重構(gòu)及心房肌組織纖維化的抑制作用。結(jié)論 天麻素可抑制陣發(fā)性房顫大鼠心房結(jié)構(gòu)重構(gòu)及心房肌組織纖維化,該機(jī)制可能與上調(diào)miR-26a-5p、抑制WNT5A表達(dá)有關(guān)。
[Key word]
[Abstract]
Objective To explore the mechanism of gastrodin in preventing and treating paroxysmal atrial fibrillation in rats model based on miR-26a-5p/WNT5A signaling pathway. Methods Rats were randomly divided into control group, model group, gastrodin (16.665、66.660 mg/kg) group, amiodarone group, gastrodin + inhibitor NC group, and gastrodin + miR-26a-5p inhibitor group, with 12 rats in each group. The induction time and duration of atrial fibrillation, LVFS, LVEF, left atrial diameter, left atrial area were detected in each group. Atrial muscle tissue fibrosis was detected by Masson staining. Immunohistochemical staining was used to indicate positive expression area of α-SMA and Collagen I in atrial muscle tissue. The level of miR-26a-5p in atrial muscle tissue was measured by qRT-PCR. Western blotting was used to detect the WNT5A experssion in atrial muscle tissue. Results Compared with the model group, the degree of atrial muscle tissue fibrosis in the gastrodin group was alleviated, the induction time of atrial fibrillation was prolonged, the duration of atrial fibrillation was shortened, LVFS and LVEF were increased, and the left atrial diameter and left atrial area were decreased (P < 0.05). The level of miR-26a-5p was significantly increased, while positive expression area of α-SMA and Collagen I positive cells was decreased, and the expression of WNT5A was also decreased in atrial muscle tissue (P < 0.05). miR-26a-5p inhibitor reversed the inhibitory effect of gastrodin on atrial structural remodeling and atrial muscle tissue fibrosis in paroxysmal atrial fibrillation rats. Conclusion Gastrodin can inhibit atrial structural remodeling and atrial muscle tissue fibrosis in rats with paroxysmal atrial fibrillation, which may be related to the upregulation of miR-26a-5p and the downregulation of WNT5A expression.
[中圖分類號]
R285.5;R286.2
[基金項(xiàng)目]
河南省醫(yī)學(xué)科技攻關(guān)計(jì)劃聯(lián)合共建項(xiàng)目(LHGJ20230738)