目的 應用祛濕止癢洗劑治療大鼠濕疹模型，考察祛濕止癢洗劑對大鼠濕疹樣皮損的藥理作用，并從免疫炎癥和皮損處菌群角度探究其治療濕疹的機制。方法 56只雌性SD大鼠，隨機分為對照組、模型組、祛濕止癢洗劑（5.5、11、22 g/L）組、地塞米松組、爐甘石洗劑組。通過記錄皮損評分、耳腫脹、瘙癢次數(shù)評價療效。蘇木精–伊紅染色法（HE）染色觀察皮膚組織形態(tài)學變化。酶聯(lián)免疫法檢測皮血清中白細胞介素（IL）-2、IL-17、IL-23及免疫球蛋白E（IgE）表達水平。血液分析檢查嗜酸性粒細胞、白細胞、中性粒細胞水平。免疫組化法檢測背部皮損組織中IL-17、IL-23陽性表達，采用16S rRNA技術(shù)測定皮損處微生物變化。結(jié)果 與模型組相比，祛濕止癢洗劑組治療后濕疹大鼠的皮膚損傷、瘙癢、耳腫脹情況明顯減輕（P＜0.05、0.01），祛濕止癢洗劑（5.5、22 g/L）組脾臟指數(shù)顯著降低（P＜0.01）。濕疹大鼠背部皮損皮膚痂皮愈合加快，炎癥細胞浸潤減輕。血清中IL-2、IL-17、IL-23、IgE水平明顯降低（P＜0.05、0.01）。祛濕止癢洗劑各劑量組血液中嗜酸性粒細胞明顯降低，背部皮損組織中IL-17、IL-23表達明顯降低（P＜0.01）。大鼠背部皮損處皮膚表面葡萄球菌屬、棒狀桿菌屬菌群絕對豐度值有明顯的降低，而乳桿菌屬有明顯的升高。結(jié)論 祛濕止癢洗劑可治療大鼠濕疹所致的瘙癢和皮膚損傷，其作用機制可能與調(diào)節(jié)免疫、炎癥反應及影響皮損處的菌群相關(guān)。

Objective To investigate the pharmacological effects of Qushi Zhiyang Lotion on eczema-like skin lesions in rats by using Qushi Zhiyang Lotion to treat eczema model in rats, and to explore its mechanism of treating eczema from the perspective of immune inflammation and skin lesion flora. Methods 56 Female SD rats were randomly divided into control group, model group, Qushi Zhiyang Lotion (5.5, 11, 22 g/L) group, dexamethasone group, and calamine lotion group. The efficacy was evaluated by skin lesion score, ear swelling and itching times. HE was used to observe the morphological changes of skin tissue. The expression levels of IL-2, IL-17, IL-23, and IgE in serum were detected by enzyme-linked immunosorbent assay. Blood analysis examined eosinophils, white blood cells, and neutrophil levels. The expression levels of IL-17 and IL-23 in dorsal skin lesions were detected by immunohistochemistry, and the microbial changes in skin lesions were determined by 16 S-rRNA technique. Results Compared with model group, the skin damage, itching and ear swelling of the eczema rats in the Qushi Zhiyang Lotion group were significantly reduced (P< 0.05, 0.01), and the spleen index in the Qushi Zhiyang Lotion (5.5, 22 g/L) group was decreased (P< 0.01). The healing of skin scabs on the back of eczema rats was accelerated, and the infiltration of inflammatory cells was reduced. The levels of IL-2, IL-17, IL-23, and IgE in serum were significantly decreased (P< 0.05, 0.01). Eosinophils in the blood decreased significantly, the expression of IL-17 and IL-23 in dorsal skin lesions was significantly decreased (P< 0.01). The absolute abundance of Staphylococcus and Corynebacterium on the skin surface of the skin lesions on the back of the rats was significantly reduced, while Lactobacillus was significantly increased. Conclusion Qushi Zhiyang Lotion can treat pruritus and skin damage caused by eczema in rats, and its mechanism may be related to the regulation of immunity, inflammatory response and the influence of flora at skin lesions.

R287.6

廣西重點研發(fā)計劃項目（桂科AB25069028）；廣西中醫(yī)藥大學“桂派中醫(yī)藥傳承創(chuàng)新團隊”項目（2022A005）