目的 研究醋艾炭炮制過程中飲片粉末色度值與高效液相色譜（HPLC）特征圖譜的相關(guān)性。方法 測定不同炮制時間醋艾炭飲片的特征圖譜和色度值（L^*、a^*、b^*、E^*），并對成分和色度值進行相關(guān)性分析和多元統(tǒng)計分析。結(jié)果 隨著炮制時間的延長，醋艾炭較艾葉特征圖譜相似度逐漸降低；峰6、峰13單位峰面積先升高后下降，其余色譜峰單位峰而積呈下降趨勢；L^*、b^*、E^*值逐新減小，a^*值趨于穩(wěn)定，ΔE^*逐漸增大。異澤蘭黃素與L^*、E^*值呈極顯著正相關(guān)，綠原酸與a^*值呈極顯著負相關(guān)，與b^*值呈極顯著正相關(guān)，棕矢車菊素與E^*值呈極顯著正相關(guān)。正交偏最小二乘法判別分析（OPLS-DA）結(jié)果顯示峰6、峰2、峰13、峰8、夏佛塔苷、咖啡酸和隱綠原酸是醋艾炭飲片炮制過程中產(chǎn)生質(zhì)量差異的主要指標。結(jié)論 不同炮制時間醋艾炭飲片的色度值與HPIC特征圖譜密切相關(guān)，為醋艾炭炮制過程化學成分動態(tài)監(jiān)測和質(zhì)量評價提供手段。

Objective To research the correlation between the chromaticity value of and HPLC characteristic chromatogram of vinegar-processed Artemisiae Argyi Folium Carbonisata (VAFC). Methods The characteristic chromatograms and chromaticity values (L^*, a^*, b^*, E^*) were determined at different processing times, and the correlation analysis and multivariate statistical analysis were carried out to analyze the relationship between chromaticity values and chemical components. Results With the prolongation of processing time, the similarity of the characteristic chromatograms of VAFC gradually decreased compared with Artemisiae Argyi Folium. The unit peak area of peak 6 and peak 13 increased first and then decreased, while other chromatographic peaks decreased. The values of L^*, b^*,and E^* decreased gradually, the values of a^* tended to be stable, and the values of ΔE^* increased gradually. There were significant positive correlations between eupatilin and L^* and E^* values, significant negative correlations between chlorogenic acid and a^* values, significant positive correlations between chlorogenic acid and b^* values, and significant positive correlations between jaceosidin and E^* values. The results of orthogonal partial least squares discrmiant analysis (OPLS-DA) showed that peak 6, peak 2, peak 13, peak 8, schaftoside, caffeic acid, and cryptochlorogenic acid were the main indexes of quality difference during the processing of VAFC. Conclusion The chromaticity values of VAFC at different processing time are closely related to the HPIC characteristic chromatograms, which provides a reference for the dynamic monitoring of chemical components and quality evaluation of VAFC during processing.

R283.1;R286.02

江西省中醫(yī)藥標準化研究項目（2023A15）