目的 建立水紅花子中多指標成分定量，結合化學計量學評價不同產地水紅花子的質量。方法 采用HPLC法測定水紅花子中原兒茶酸、兒茶素、異葒草素、葒草素、花旗松素、木犀草素、槲皮素、山柰酚、圣草酚、白樺脂酸和β-谷甾醇，并檢測醇溶性浸出物、總灰分和酸不溶性灰分，采用主成分分析、因子分析、正交偏最小二乘法-判別分析、熵權-TOPSIS對檢測結果分析。結果 18批水紅花子樣品自動聚為3類，呈現(xiàn)地域性分布。兒茶素、花旗松素、原兒茶酸、槲皮素、山柰酚、β-谷甾醇和異葒草素7個成分可作為引起不同產地水紅花子之間質量差異的主要變量，是質量差異標志物。黑龍江、遼寧和吉林產地的水紅花子整體質量較好，其中遼寧西豐縣的樣品質量最優(yōu)，廣西和四川產的水紅花子質量較差。結論 所建立的多組分定量和化學計量學控制模式簡單、易推廣，可用于水紅花子的內在質量評價。

Objective To establish an HPLC multi-index component quantification method for Polygoni Orientalis Fructus, and to evaluate the quality of Polygoni Orientalis Fructus from different areas by chemometrics. Methods Simultaneous determination of protocatechuic acid, catechin, isoorientin, orientin, taxifolin, luteolin, quercetin, kaempferol, eriodictyol, betulinic acid, and β- sitosterol in Polygoni Orientalis Fructus were carried on by HPLC method. Alcohol-soluble extractive, total ash, and acid-insoluble ash were also detected. PCA, FA, OPLS-DA, and EW-TOPSIS methods were used to analyze the results. Results 18 Batches of Polygoni Orientalis Fructus samples were automatically clustered into 3 categories, showing a regional distribution. Seven components, including catechins, taxifolin, protocatechuic acid, quercetin, kaempferol, β-sitosterol, and isoorientin, could serve as the main variables causing quality differences in Polygoni Orientalis Fructus from different areas and were indicators of quality differences. The overall quality of samples from Heilongjiang, Liaoning, and Jilin Province production areas were good, with the sample quality from Xifeng County, Liaoning Province being the best, while the samples from Guangxi and Sichuan Province had poor quality. Conclusion The multi-index component quantification method and chemometric control mode established is simple and easy to generalize, and can be used for the internal quality evaluation for Polygoni Orientalis Fructus.

R286.02

河南省高等學校重點科研指導計劃項目（20B550007）