目的 建立草紅藤的UPLC指紋圖譜，結(jié)合化學(xué)模式識別分析手段評價不同產(chǎn)地的草紅藤藥材質(zhì)量差異。方法 采用UPLC法建立草紅藤藥材指紋圖譜，進行相似度評價、系統(tǒng)聚類分析、主成分分析（PCA）和正交偏最小二乘法判別分析（OPLS-DA）。結(jié)果 20批草紅藤藥材UPLC指紋圖譜共確定9個共有峰，指認出3個色譜峰，分別是二氫楊梅素、楊梅素和花旗松素，相似度均大于0.9。聚類分析和主成分分析將20批草紅藤藥材分為3類，經(jīng)OPLS-DA篩選出3個差異成分。結(jié)論 建立的草紅藤藥材UPLC指紋圖譜方法簡便，重現(xiàn)性良好，同時結(jié)合化學(xué)模式識別分析，可為草紅藤的質(zhì)量控制和品質(zhì)提升提供參考。

Objective To establish an UPLC fingerprint spectrum of Shuteria involucrata (Wall.) Wight et Arn var. villosa (Pampan.) Ohashi, and evaluate the quality differences of S. involucrata var. villosa from different origins using chemical pattern recognition analysis methods. Methods UPLC method was used to establish the fingerprint of S. involucrata var. villosa and the fingerprint data was analyzed by similarity evaluation, systematic clustering analysis, PCA, and OPLS-DA. Results A total of 9 common peaks were marked in the UPLC fingerprint spectra of 20 batches of S. involucrata var. villosa, and 3 chromatographic peaks were identified, namely dihydromyricetin, myricetin, and taxifolin. The similarity was greater than 0.9. According to cluster analysis and principal component analysis, the 20 batches of samples were divided into three groups. And three differentially expressed components were screened using OPLS-DA. Conclusion The established UPLC fingerprint method is simple and has good reproducibility, and combined with chemical pattern recognition analysis, which can provide reference for the quality control of S. involucrata var. villosa.

R286.02