目的 建立貓爪草中成分的HPLC法，利用化學(xué)計(jì)量學(xué)、加權(quán)優(yōu)劣解距離法、灰色關(guān)聯(lián)度融合模型評價(jià)不同產(chǎn)地貓爪草質(zhì)量。方法 建立HPLC外標(biāo)法測定貓爪草中沒食子酸、咖啡酸、鞣花酸、3,4-二甲氧基苯甲酸、原白頭翁素、小毛茛內(nèi)酯、穗花杉雙黃酮、7-O-甲基圣草酚、白頭翁素、豆甾醇、β-谷甾醇，并檢測浸出物、總灰分和酸不溶性灰分，結(jié)合化學(xué)計(jì)量學(xué)、加權(quán)優(yōu)劣解距離法與灰色關(guān)聯(lián)度融合模型對貓爪草質(zhì)量進(jìn)行綜合評價(jià)。結(jié)果 貓爪草中活性成分批次間質(zhì)量分?jǐn)?shù)差異較大。18批貓爪草樣品被分為3個(gè)類群，對貓爪草分類和質(zhì)量貢獻(xiàn)較大的成分有小毛茛內(nèi)酯、穗花杉雙黃酮、沒食子酸、咖啡酸和β-谷甾醇。不同產(chǎn)地貓爪草樣品質(zhì)量存在顯著差異，河南、安徽和江蘇省產(chǎn)貓爪草的質(zhì)量較優(yōu)。結(jié)論 所建方法簡單、科學(xué)，為貓爪草的質(zhì)量控制提供參考。

Objective To establish an HPLC method for components in Ranunculi Ternati Radix, and to evaluate the quality of Ranunculi Ternati Radix from different origins by using the chemometrics, weighted TOPSIS, and grey correlation degree fusion model. Method The contents of gallic acid, caffeic acid, ellagic acid, 3,4-dimethoxybenzoic acid, protoanemonin, ternatolide,amentoflavone, 7-O-methyleriodictyol, anemonine, stigmasterol, and β-sitosterol were determined by HPLC-external standard method. The extract, total ash, and acid-insoluble ash were detected. The quality of Ranunculi Ternati Radix was analyzed by chemometrics, weighted TOPSIS, and grey correlation degree fusion model. Results There were significant differences of active ingredients contents in Ranunculi Ternati Radix between different batches. The 18 batches of Ranunculi Ternati Radix samples were divided into three groups, and the components that contributed significantly to the classification and quality of Ranunculi Ternati Radix included ternatolide, 7-amentoflavone, gallic acid, caffeic acid, and β-sitosterol. There were significant differences in the quality of Ranunculi Ternati Radix samples from different origins, with Henan, Anhui, and Jiangsu Provinces producing better quality Ranunculi Ternati Radix. Conclusion The established method is simple and scientific, providing reference for the quality control of Ranunculi Ternati Radix.

R286.02

河南省高等學(xué)校重點(diǎn)科研指導(dǎo)計(jì)劃項(xiàng)目(20B460010)