目的 評(píng)估黃芩素在大鼠體內(nèi)抑制深靜脈血栓形成的活性并探索作用機(jī)制。方法 將72只雄性SD大鼠隨機(jī)分為對(duì)照組、模型組、華法林（0.27 mg/kg）組與黃芩素（5、10、20 mg/kg）組。除對(duì)照組以外，每日ig給藥1次，連續(xù)給藥4 d。應(yīng)用大鼠下腔靜脈完全瘀滯血栓形成模型造模，測(cè)定各組大鼠下腔靜脈內(nèi)形成的血栓質(zhì)量；通過HE染色病理切片觀察下腔靜脈的形態(tài)與靜脈壁厚度；ELISA測(cè)定大鼠血漿P-選擇素和白細(xì)胞介素-6（IL-6）水平，以及大鼠血小板糖蛋白IIb/IIIa含量；同時(shí)測(cè)定出血時(shí)間與凝血時(shí)間。結(jié)果 與模型組比較，黃芩素10、20 mg/kg組形成的血栓質(zhì)量顯著降低，下腔靜脈血管壁厚度減小，血漿P-選擇素水平顯著降低（P＜0.05、0.01）；與模型組比較，黃芩素20 mg/kg組IL-6水平和血小板GPIIb/IIIa含量顯著降低（P＜0.01）；與模型組比較，黃芩素5、10、20 mg/kg組大鼠尾出血時(shí)間顯著延長(zhǎng)（P＜0.05、0.001）。結(jié)論 黃芩素具有抑制體內(nèi)深靜脈血栓形成的活性，其作用機(jī)制可能與其降低體內(nèi)炎癥反應(yīng)相關(guān)因子，以及影響血小板的功能有關(guān)。

Objective To evaluate the inhibition effect of baicalein on deep vein thrombosis in rats, and explore the mechanism. Methods 72 Male SD rats were randomly divided into the control group, model group, warfarin (0.27 mg/kg) group, and baicalein group (5, 10, 20 mg/kg). Except for the control group, each group was administered by gavage once daily for 4 days. The rat complete stasis model of inferior vena cava thrombosis was used, and the weight of thrombus formed in the inferior vena cava of each group was determined. The morphology and venous wall thickness of the inferior vena cava were observed by HE stained pathological sections. The plasma levels of P-selectin and IL-6, and the content of platelets GPIIb/IIIa in rats were determined by ELISA. Meanwhile, the rat tail bleeding time and clotting time were measured. Results Compared with the model group, baicalein 10 and 20 mg/kg group reduced the weight of thrombus formed in inferior vena cava, the thickness of the inferior vena cava vessel wall, and the P-selectin level (P< 0.05, 0.01). Compared with the model group, baicalein 20 mg/kg group reduced the IL-6 level and the content of glycoprotein IIbIIIa on platelet (P< 0.01). Compared with the model group, baicalein 5, 10, and 20 mg/kg group prolonged the bleeding time of rat tail (P< 0.05, 0.001). Conclusion Baicalein has the activity of inhibiting venous thrombosis in vivo, and its mechanism may be related to its reduction of inflammatory factors, and affecting the function of platelets.

R286.2

北京市衛(wèi)生健康科技成果和適宣技術(shù)推廣項(xiàng)目（BHTPP2022049）;首都衛(wèi)生發(fā)展科研專項(xiàng)項(xiàng)目（2022-2Z-20215）