目的 建立RP-HPLC法測定眩暈寧顆粒中3，5-O-二咖啡?；鼘幩帷?3-乙酰澤瀉醇B和β-蛻皮甾酮的測定方法。方法 采用Capcell Pak UG C₁₈色譜柱（250 mm×4.6 mm，5 μm）；流動相為乙腈–0.5%磷酸溶液，梯度洗脫；檢測波長：348 nm（3，5-O-二咖啡?；鼘幩幔?、250 nm（23-乙酰澤瀉醇B）、208 nm（β-蛻皮甾酮）；體積流量：1.0 mL/min；柱溫：28℃；進(jìn)樣量：10 μL。結(jié)果 3，5-O-二咖啡?；鼘幩?、β-蛻皮甾酮和23-乙酰澤瀉醇B分別在0.076 7～7.670 0、0.098 3～9.830 0、0.019 7～1.970 0 μg/mL線性良好，平均回收率分別為100.05%、98.33%、99.42%，RSD值分別為0.7%、1.2%、1.3%。結(jié)論 方法具有前處理簡單、分析時間短、檢測結(jié)果準(zhǔn)確等優(yōu)點，適用于眩暈寧顆粒的質(zhì)量控制。

Objective To establish an RP-HPLC method for the determination of 3,5-O-dicaffeyl quinic acid, 23-acetylalisol B, and β-ecdysteroid in Xuanyunning Granules. Methods The separation was carried out on Capcell Pak UG C₁₈ column (250 mm×4.6 mm, 5 μm). The mobile phase was acetonitrile-0.5% phosphoric acid solution with gradient elution. The detection wavelength was set at 348 nm (3,5-O-dicaffeyl quinic acid), 250 nm (23-acetylalisol B), and 208 nm (β-ecdysterone). The flow rate was 1.0 mL/min, temperature of column was set at 28℃, and the volume of injection was 10 μL. Results 3,5-O-dicaffeyl quinic acid, 23-acetylalisol B, and β-ecdysteroid showed good linear relationships in concentration ranges of 0.076 7-7.670 0, 0.098 3-9.830 0, and 0.019 7-1.970 0 μg/mL, and their average recoveries were 100.05%, 98.33%, and 99.42% with RSD values of 0.7%, 1.2%, and 1.3%, respectively. Conclusion The method has the advantages of simple pretreatment, short analysis time, and accurate detection results, and is suitable for the quality control of Xuanyunning Granules.

R286.02

安徽醫(yī)科大學(xué)校科研基金項目（（2020xkj241）