目的 建立HPLC法同時(shí)測(cè)定艽龍膠囊中馬錢(qián)苷酸、山梔苷甲酯、6'-O-β-D-葡萄糖基龍膽苦苷、獐牙菜苦苷、龍膽苦苷、獐牙菜苷、異葒草苷、異牡荊黃素的方法。方法 采用Intersil C₁₈色譜柱（250 mm×4.6 mm，5 μm）；流動(dòng)相：乙腈–0.2%磷酸溶液，梯度洗脫；檢測(cè)波長(zhǎng)：254 nm；體積流量：0.8 mL/min；柱溫：25℃；進(jìn)樣量：10 μL。結(jié)果 馬錢(qián)苷酸、山梔苷甲酯、6'-O-β-D-葡萄糖基龍膽苦苷、獐牙菜苦苷、龍膽苦苷、獐牙菜苷、異葒草苷、異牡荊黃素分別在21.20～2 120.00、3.20～320.00、15.00～1 500.00、5.10～51.00、39.00～3 900.00、4.20～420.00、2.20～220.00、1.10～110.00 μg/mL與峰面積呈良好的線性關(guān)系；平均回收率分別為99.37%、97.28%、96.49%、99.37%、97.28%、96.49%、99.37%、97.28%，RSD值分別為1.64%、1.16%、1.85%、1.64%、1.16%、1.85%、1.64%、1.16%。結(jié)論 方法簡(jiǎn)便、重復(fù)性好，結(jié)果準(zhǔn)確可靠，可為進(jìn)一步完善艽龍膠囊的質(zhì)量評(píng)價(jià)方法提供參考依據(jù)。

Objective To develop an HPLC method for simultaneous determination of loganic acid, shanzhiside methyl ester, 6'-O-β-D-glucosylgentiopicroside, swertiamarin, gentiopicroside, sweroside, isoorientin, and isovitexin in Jiaolong Capsules. Methods The separation was carried out on Intersil C₁₈ column (250 mm×4.6 mm, 5 μm). The mobile phase was acetonitrile-0.2% phosphoric acid solution with gradient elution. The detection wavelength was set at 254 nm. The volume flow was 0.8 mL/min, the column temperature was 25℃, and the injection volume was 10 μL. Results Loganic acid, shanzhiside methyl ester, 6'-O-β-D-glucosylgentiopicroside, swertiamarin, gentiopicroside, sweroside, isoorientin, and isovitexin showed good linear relationships within the ranges of 21.20-2 120.00, 3.20-320.00, 15.00-1 500.00, 5.10-51.00, 39.00-3 900.00, 4.20-420.00, 2.20-220.00, 1.10-110.00 μg/mL, and their average recoveries were 99.37%, 97.28%, 96.49%, 99.37%, 97.28%, 96.49%, 99.37%, and 97.28% with RSD values of 1.64%, 1.16%, 1.85%, 1.64%, 1.16%, 1.85%, 1.64%, and 1.16%, respectively. Conclusion The method is simple, reproducible, and the results are accurate and reliable, which can provide a reference for further improving the quality evaluation method of Jiaolong Capsules.

R286.02

陜西省科學(xué)技術(shù)研究發(fā)展計(jì)劃項(xiàng)目（2015SF2-08-01）