目的 通過網(wǎng)絡(luò)藥理學(xué)方法探究黃芪治療肺結(jié)核的作用機(jī)制。方法 運(yùn)用TCMSP數(shù)據(jù)庫(kù)篩選黃芪的活性成分及其作用靶點(diǎn)；借助GeneCard數(shù)據(jù)庫(kù)篩選肺結(jié)核疾病靶點(diǎn)，并獲得交集靶點(diǎn)；利用STRING數(shù)據(jù)庫(kù)構(gòu)建靶點(diǎn)蛋白相互作用網(wǎng)絡(luò)；將交集靶點(diǎn)進(jìn)行基因本體（gene ontology，GO）和京都基因與基因組百科全書（kyoto encyclopedia of genes and genomes，KEGG）富集分析。結(jié)果 篩選得到黃芪的活性成分20個(gè)，活性成分主要包括槲皮素、異鼠李素、異黃酮等，其治療肺結(jié)核的相關(guān)靶點(diǎn)為TNF、RELA、IL-6、AKT1、MAPK1、MAPK14、IL-1B、MAPK8、IL-10、IFNG、STAT1等；KEGG分析結(jié)果表明黃芪可能通過干預(yù)Toll樣受體信號(hào)通路、結(jié)核病通路、T細(xì)胞受體信號(hào)通路及TNF信號(hào)通路等發(fā)揮治療肺結(jié)核的作用。結(jié)論 黃芪治療肺結(jié)核具有多成分–多靶點(diǎn)–多通路的特點(diǎn)，多與炎癥、免疫機(jī)制相關(guān)，為黃芪的實(shí)驗(yàn)研究和臨床合理用藥提供科學(xué)依據(jù)。

Obtective To investigate the pharmacology mechanism of Astragali Radix for pulmonary tuberculosis based on network pharmacology.Methods The TCMSP database was used to screen the active ingredients of Astragali Radix and their targets, the GeneCard database was used to screen the pulmonary tuberculosis targets and obtain the intersecting targets, the STRING database was used to construct the target protein interaction network, the intersecting targets were subjected to GO and KEGG enrichment analysis.Results Twenty active components of Astragali Radix were screened, the active ingredients mainly include quercetin, isorhamnetin, and isoflavone, and their relevant targets for the treatment of pulmonary tuberculosis were TNF, RELA, IL-6, AKT1, MAPK1, MAPK14, IL-1B, MAPK8, IL-10, IFNG, STAT1, etc. The results of KEGG analysis indicated that Astragali Radix may interfere with the Toll-like receptor signaling pathway, tuberculosis pathway, T-cell receptor signaling pathway and TNF signaling pathway to play a role in treatment of pulmonary tuberculosis.Conclusion Astragali Radix has multi-component-multi-target-multi-pathway characteristics in the treatment of pulmonary tuberculosis, mostly related to inflammation and immune mechanism, which provides scientific basis for experimental research and rational clinical use of astragalus.

R285.5

昆明市衛(wèi)生科技人才培養(yǎng)項(xiàng)目（2020-SW[后備]-60）；昆明市衛(wèi)生健康委員會(huì)衛(wèi)生科研課題項(xiàng)目（2021-16-01-0010）