目的 利用生物分子網(wǎng)絡(luò)探討桑白皮湯治療慢性阻塞性肺疾?。–OPD）的作用機(jī)制。方法 通過(guò)中藥系統(tǒng)藥理數(shù)據(jù)庫(kù)和分析平臺(tái)（TCMSP）收集桑白皮湯的化學(xué)成分和成分對(duì)應(yīng)的靶點(diǎn)，并利用Cytoscape軟件構(gòu)建中藥-成分-靶點(diǎn)網(wǎng)絡(luò)；通過(guò)GeneCards、TTD和DisGeNET數(shù)據(jù)庫(kù)獲取與COPD相關(guān)的靶點(diǎn)，對(duì)成分靶點(diǎn)和疾病靶點(diǎn)取交集，利用String和Cytoscape構(gòu)建交集靶點(diǎn)網(wǎng)絡(luò)；利用DAVID數(shù)據(jù)庫(kù)對(duì)交集靶點(diǎn)進(jìn)行KEGG和GO富集分析。結(jié)果 篩選得到109個(gè)化學(xué)成分，230個(gè)成分靶點(diǎn)，COPD相關(guān)靶點(diǎn)1 220個(gè)，成分和疾病的交集靶點(diǎn)119個(gè)。KEGG分析表明桑白皮湯可能通過(guò)PI3K-Akt信號(hào)通路、TNF信號(hào)通路、MAPK信號(hào)通路、NF-κB信號(hào)通路、HIF-1信號(hào)通路、凋亡相關(guān)通路等發(fā)揮治療COPD的作用。GO分析顯示桑白皮湯主要涉及炎癥反應(yīng)、細(xì)胞增殖、細(xì)胞凋亡、RNA轉(zhuǎn)錄、基因表達(dá)等。結(jié)論 通過(guò)構(gòu)建生物分子網(wǎng)絡(luò)，揭示了桑白皮湯治療COPD的分子機(jī)制，為桑白皮湯的后續(xù)研究提供了理論依據(jù)。

Objective To explore the mechanism of Sangbaipi Decoction in treatment of COPD through biological network. Methods The chemical components and corresponding targets of Sangbaipi Decoction were collected by TCMSP, and the network of herb-ingredient-target was constructed by Cytoscape software. The targets related to COPD were obtained from GeneCards, TTD, and DisGeNET databases. The intersection targets of components and disease were integrated.Target network of intersection targets was constructed using String online database and Cytoscape software. The results of KEGG and GO enrichment analysis of intersection targets was obtained from DAVID database. Results A total of 109 active components and 230 corresponding targets were obtained. Furthermore, 1 220 COPD related targets and 119 intersection targets were obtained. KEGG analysis revealed that Sangbaipi Decoction might treat COPD through PI3K-Akt signaling pathway, TNF signaling pathway, MAPK signaling pathway, NF-κB signaling pathway, HIF-1 signaling pathway, and Apoptosis-related pathway. GO analysis demonstrated that Sangbaipi Decoction was mainly involved in inflammatory reaction, cell proliferation, apoptosis, RNA transcription, gene expression. Conclusion The molecular mechanism of Sangbaipi Decoction in treatment of COPD were discussed, which might provide a theoretical basis for further exploration.

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國(guó)家自然科學(xué)基金資助項(xiàng)目（81803561）；成都市金牛區(qū)醫(yī)學(xué)會(huì)科研課題（JNKY2021-06）