目的 利用網(wǎng)絡(luò)藥理學(xué)方法探討腫節(jié)風(fēng)治療前列腺癌的可能作用機(jī)制。方法 通過(guò)TCMSP、Uniprot、GeneCards在線數(shù)據(jù)庫(kù)篩選腫節(jié)風(fēng)活性化合物治療前列腺癌的潛在靶點(diǎn)，利用Cytoscape軟件構(gòu)建前列腺癌-腫節(jié)風(fēng)-靶點(diǎn)網(wǎng)絡(luò)圖，然后結(jié)合STRING數(shù)據(jù)庫(kù)和Cytoscape軟件構(gòu)建蛋白質(zhì)相互作用網(wǎng)絡(luò)；最后利用R軟件對(duì)靶點(diǎn)進(jìn)行GO分類富集分析和KEGG通路富集分析，預(yù)測(cè)其作用機(jī)制，并構(gòu)建靶點(diǎn)通路網(wǎng)絡(luò)。結(jié)果 從腫節(jié)風(fēng)中篩選出活性成分10個(gè)，其與前列腺癌共同作用靶點(diǎn)170個(gè)，GO功能分析發(fā)現(xiàn)靶點(diǎn)主要與膜伐、對(duì)氧水平、泛素樣蛋白連接酶結(jié)合等有關(guān)。KEGG通路分析發(fā)現(xiàn)腫節(jié)風(fēng)治療前列腺癌主要與糖尿病并發(fā)癥中的AGE-RAGE信號(hào)通路、前列腺癌（PCa）、流體剪切應(yīng)力（FSS）與動(dòng)脈粥樣硬化（AS）等通路密切相關(guān)。結(jié)論 腫節(jié)風(fēng)活性成分可能通過(guò)多靶點(diǎn)、多通路達(dá)到治療及輔助治療前列腺癌的目的。

Objective To explore the possible mechanism of Glabrous Sarcandra Herb in treatment of prostate cancer by network pharmacology. Methods The potential targets of active compounds of Glabrous Sarcandra Herb in treatment of prostate cancer were screened by TCMSP, Uniprot and GeneCards online databases. Cytoscape software was used to construct a network map of prostate cancer tumor targets, and then STRING database and Cytoscape software were combined to construct a protein interaction network. R software was used to perform GO classification enrichment analysis and KEGG pathway enrichment analysis on the targets. mechanism of Glabrous Sarcandra Herb in treatment of prostate cancer were predicted and a target pathway network was constructed. Results Ten active ingredients were screened out from Glabrous Sarcandra Herb, 170 targets that interacted with prostate cancer. GO function analysis revealed that the targets were mainly related to membrane cutting, para-oxygen level, and ubiquitin-like protein ligase binding. KEGG pathway analysis found that the AGE-RAGE signaling pathway, prostate cancer (PCa), fluid shear stress (FSS) and atherosclerosis (AS) pathways in the complications of diabetes were mainly related to Glabrous Sarcandra Herb in treatment of prostate cancer. Conclusion The active ingredients of Glabrous Sarcandra Herb may achieve the purpose of treatment and adjuvant treatment of prostate cancer through multiple targets and multiple pathways.

R285.5

國(guó)家自然科學(xué)基金資助項(xiàng)目（81871155）